Weber Kassandra, Schilling Joel D
Diabetic Cardiovascular Disease Center, Washington University School of Medicine, St. Louis, MO 63110, USA ; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
Diabetic Cardiovascular Disease Center, Washington University School of Medicine, St. Louis, MO 63110, USA ; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA ; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Int J Inflam. 2014;2014:154936. doi: 10.1155/2014/154936. Epub 2014 Dec 23.
Lysosomes play a critical role in the degradation of both extracellular and intracellular material. These dynamic organelles also contribute to nutrient sensing and cell signaling pathways. Macrophages represent a heterogeneous group of phagocytic cells that contribute to tissue homeostasis and inflammation. Recently, there has been a renewed interest in understanding the role of macrophage autophagy and lysosome function in health and disease. Thioglycollate-elicited peritoneal and bone marrow-derived macrophages are commonly used ex vivo systems to study primary macrophage function. In this study, we reveal dramatic baseline differences in the lysosome morphology and function between these macrophage populations and provide evidence that these differences can be functionally relevant. Our results provide important insights into the diversity of lysosomes in primary macrophages and illustrate the importance of accounting for this in data interpretation.
溶酶体在细胞外和细胞内物质的降解中起着关键作用。这些动态细胞器还参与营养感知和细胞信号通路。巨噬细胞是一类异质性的吞噬细胞，对组织稳态和炎症有贡献。最近，人们对了解巨噬细胞自噬和溶酶体功能在健康和疾病中的作用重新产生了兴趣。巯基乙酸诱导的腹腔巨噬细胞和骨髓来源的巨噬细胞是常用的体外系统，用于研究原代巨噬细胞功能。在本研究中，我们揭示了这些巨噬细胞群体之间溶酶体形态和功能的显著基线差异，并提供证据表明这些差异可能具有功能相关性。我们的结果为原代巨噬细胞中溶酶体的多样性提供了重要见解，并说明了在数据解释中考虑这一点的重要性。
