Zuo Tao, Cao Lu, Li Xuemin, Zhang Qi, Xue Changhu, Tang Qingjuan
a College of Food Science and Engineering , Ocean University of China , Qingdao , China.
Nutr Cancer. 2015;67(2):364-71. doi: 10.1080/01635581.2015.989369. Epub 2015 Jan 14.
Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alterations in the functioning of the intestinal barrier, due to the potential damage induced by anticancer drugs on the epithelial tight junctions and adheren junction. We aimed to study the protective effect of dietary polysaccharides on chemotherapy-induced injury in the epithelial cells. In the current study, using mice that were intraperitoneally injected with 50 mg/kg cyclophosphamide for 2 days, we reveal that polysaccharides from the ink of Ommastrephes bartrami (OBP) enhanced the mRNA and protein expression levels of Occludin, zonulae occluden (ZO)-1, and E-cadherin. Immunohistochemistry staining of ZO-1 and E-cadherin confirmed the increase in the mRNA and protein levels. OBP also remarkably enhanced the mRNA expression of other tight junction proteins, ZO-2, ZO-3, claudin-2, and cingulin. Our results may have important implications in host defense, especially the immunopotentiation function of OBP on the cyclophosphamide-induced epithelial cell injury, as well as intestinal disorders involving inflammation and infection.
胃肠道黏膜炎和化疗引起的感染与肠道屏障功能的改变有关，这是由于抗癌药物对上皮紧密连接和黏附连接可能造成的损害。我们旨在研究膳食多糖对化疗诱导的上皮细胞损伤的保护作用。在本研究中，我们使用腹腔注射50 mg/kg环磷酰胺连续2天的小鼠，发现柔鱼墨多糖（OBP）可提高闭合蛋白、闭合小带蛋白（ZO）-1和E-钙黏蛋白的mRNA和蛋白表达水平。ZO-1和E-钙黏蛋白的免疫组织化学染色证实了mRNA和蛋白水平的增加。OBP还显著提高了其他紧密连接蛋白ZO-2、ZO-3、claudin-2和cingulin的mRNA表达。我们的结果可能对宿主防御具有重要意义，尤其是OBP对环磷酰胺诱导的上皮细胞损伤的免疫增强作用，以及对涉及炎症和感染的肠道疾病的影响。
