Cha Se-Yeoun, Seo Hye-Suk, Wei Bai, Kang Min, Roh Jae-Hee, Yoon Ran-Hee, Kim Ji-Hyuk, Jang Hyung-Kwan
1 Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University, 79 Gobongro, Iksan, 570-752, South Korea.
J Wildl Dis. 2015 Apr;51(2):341-7. doi: 10.7589/2014-05-128. Epub 2015 Jan 14.
We conducted surveillance for Riemerella anatipestifer (RA) in wild birds along the East Asian-Australasian flyway in South Korea. Detected RA were characterized by serotype, antibiotic susceptibility, and sequence analysis of the 16S rRNA gene. We collected 944 wild birds of 34 species from 19 of South Korea's major migratory wild bird habitats between 2011 and 2012. We identified RA by PCR and rRNA gene sequence in 71/102 (69.6%) pharyngeal swabs and 19/944 (2.0%) cloacal swabs of wild birds. Most RA positives (71/75 [95%] pharyngeal and 19/704 [(2.6%] cloacal) were from three duck species (family Anatidae): Mallard Duck (Anas platyrhynchos), Northern Pintail (Anas acuta), and Spot-billed Duck (Anas poecilorhyncha). Thirty-three RA isolates obtained and examined were highly resistant to aminoglycosides: kanamycin (100%), gentamicin (94%), amikacin (91%), neomycin (88%), and streptomycin (82%). Six isolates were identified as serotype 4 by agar gel precipitation. Serotypes 1 and 7, which are known virulent serotypes, were also identified in three isolates from wild duck species.
我们在韩国东亚-澳大拉西亚候鸟迁徙路线沿线的野生鸟类中开展了鸭疫里默氏菌（RA）监测。对检测到的RA进行了血清型、抗生素敏感性及16S rRNA基因序列分析。2011年至2012年期间，我们从韩国19个主要野生鸟类迁徙栖息地收集了34种944只野生鸟类。我们通过PCR和rRNA基因序列在71/102（69.6%）的野生鸟类咽拭子和19/944（2.0%）的泄殖腔拭子中鉴定出RA。大多数RA阳性样本（71/75[95%]为咽拭子，19/704[2.6%]为泄殖腔拭子）来自三种鸭科鸟类：绿头鸭（Anas platyrhynchos）、针尾鸭（Anas acuta）和斑嘴鸭（Anas poecilorhyncha）。对获得并检测的33株RA分离株进行检测，发现它们对氨基糖苷类抗生素具有高度耐药性：对卡那霉素耐药率为100%、庆大霉素为94%、阿米卡星为91%、新霉素为88%、链霉素为82%。通过琼脂凝胶沉淀法鉴定出6株为血清型4。在从野鸭物种分离出的3株菌株中还鉴定出了已知的强毒血清型1和7。
