Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna, Philippines.
College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea.
PLoS One. 2020 Nov 11;15(11):e0242198. doi: 10.1371/journal.pone.0242198. eCollection 2020.
3,3'-Diindolylmethane (DIM) is found in cruciferous vegetables and is used to treat various inflammatory diseases because of its potential anti-inflammatory effects. To investigate effects of DIM in Riemerella anatipestifer-infected ducks which induce upregulation of inflammatory cytokines, ducks were treated orally with DIM at dose of 200 mg/kg/day and infected the following day with R. anatipestifer. Infected and DIM-treated ducks exhibited 14% increased survival rate and significantly decreased bacterial burden compared to infected untreated ducks. Next, the effect on the expression level of inflammatory cytokines (interleukin [IL]-17A, IL-17F, IL-6, IL-1β) of both in vitro and in vivo DIM-treated groups was monitored by quantitative reverse-transcription PCR (qRT-PCR). Generally, the expression levels of the cytokines were significantly reduced in DIM-treated splenic lymphocytes stimulated with killed R. anatipestifer compared to stimulated untreated splenic lymphocytes. Similarly, the expression levels of the cytokines were significantly reduced in the spleens and livers of DIM-treated R. anatipestifer-infected ducks compared to infected untreated ducks. This study demonstrated the ameliorative effects of DIM in ducks infected with R. anatipestifer. Thus, DIM can potentially be used to prevent and/or treat R. anatipestifer infection via inhibition of inflammatory cytokine expression.
3,3'-二吲哚甲烷（DIM）存在于十字花科蔬菜中，由于其潜在的抗炎作用，被用于治疗各种炎症性疾病。为了研究 DIM 对传染性鼻炎鸭的影响，该研究诱导鸭产生炎症细胞因子上调，用 DIM 以 200mg/kg/天的剂量经口处理鸭，次日感染鸭传染性鼻炎病菌。与未感染未经处理的鸭相比，感染并接受 DIM 治疗的鸭的存活率提高了 14%，细菌载量显著降低。接下来，通过定量逆转录 PCR（qRT-PCR）监测体外和体内 DIM 处理组的炎症细胞因子（白细胞介素[IL]-17A、IL-17F、IL-6、IL-1β）表达水平的影响。一般来说，与未经刺激的脾脏淋巴细胞相比，经 DIM 处理的受杀死鸭传染性鼻炎病菌刺激的脾脏淋巴细胞中细胞因子的表达水平显著降低。同样，与未经感染处理的鸭相比，受感染鸭传染性鼻炎病菌感染的鸭的脾脏和肝脏中细胞因子的表达水平显著降低。本研究证明了 DIM 对感染鸭传染性鼻炎病菌的改善作用。因此，通过抑制炎症细胞因子的表达，DIM 可能具有预防和/或治疗鸭传染性鼻炎病菌感染的潜力。
