Hussain Javid, Liu Yan, Lopes Wilson A, Druzian Janice I, Souza Carolina O, Carvalho Gilson C, Nascimento Iracema A, Liao Wei
LABIOMAR, Institute of Biology Federal University of Bahia, Salvador, Brazil.
Appl Biochem Biotechnol. 2015 Mar;175(6):3048-57. doi: 10.1007/s12010-015-1486-5. Epub 2015 Jan 15.
Three lipid extraction methods of hexane Soxhlet (Sox-Hex), Halim (HIP), and Bligh and Dyer (BD) were applied on freeze-dried (FD) and oven-dried (OD) Chlorella vulgaris biomass to evaluate their effects on lipid yield, fatty acid profile, and algal biodiesel quality. Among these three methods, HIP was the preferred one for C. vulgaris lipid recovery considering both extraction efficiency and solvent toxicity. It had the highest lipid yields of 20.0 and 22.0% on FD and OD biomass, respectively, with corresponding neutral lipid yields of 14.8 and 12.7%. The lipid profiling analysis showed that palmitic, oleic, linoleic, and α-linolenic acids were the major fatty acids in the algal lipids, and there were no significant differences on the amount of these acids between different drying and extraction methods. Correlative models applied to the fatty acid profiles concluded that high contents of palmitic and oleic acids in algal lipids contributed to balancing the ratio of saturated and unsaturated fatty acids and led to a high-quality algal biodiesel.
采用正己烷索氏提取法（Sox-Hex）、哈利姆法（HIP）和布利格-戴尔法（BD）这三种脂质提取方法，对冷冻干燥（FD）和烘干（OD）的小球藻生物质进行处理，以评估它们对脂质产量、脂肪酸组成以及藻类生物柴油质量的影响。在这三种方法中，综合提取效率和溶剂毒性考虑，HIP是小球藻脂质提取的首选方法。它对冷冻干燥和烘干生物质的脂质产量分别最高，为20.0%和22.0%，相应的中性脂质产量分别为14.8%和12.7%。脂质成分分析表明，棕榈酸、油酸、亚油酸和α-亚麻酸是藻类脂质中的主要脂肪酸，不同干燥和提取方法之间这些酸的含量没有显著差异。应用于脂肪酸组成的相关模型得出结论，藻类脂质中高含量的棕榈酸和油酸有助于平衡饱和脂肪酸和不饱和脂肪酸的比例，并产生高质量的藻类生物柴油。
