Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea.
Bioresour Technol. 2012 Apr;109:312-5. doi: 10.1016/j.biortech.2011.04.064. Epub 2011 May 1.
Lipids from algal biomass were extracted using mixtures of ionic liquids (ILs) and methanol, and fatty acid profiles of the extracted lipids were characterized in this work. Mixtures of ILs and methanol successfully dissolved biomass leaving lipids insoluble. The total contents of lipids extracted from commercial and cultivated Chlorella vulgaris were 10.6% and 11.1%, respectively, by the conventional Bligh and Dyer's method, while a mixture of [Bmim][CF(3)SO(3)] and methanol extracted 12.5% and 19.0% of the lipids, respectively. Multi-parameter regression by the linear solvation energy relationship showed that dipolarity/polarizability and hydrogen bond acidity of ILs are more important than their hydrogen bond basicity for effectively extracting lipids from algal biomass. Fatty acid profiles of the lipids extracted using IL-methanol mixtures showed that C16:0, C16:1, C18:2, and C18:3 fatty acids were dominant. This suggests that the lipids extracted from C. vulgaris can be used as a source of biodiesel production.
本工作采用离子液体（ILs）和甲醇的混合物从藻类生物质中提取脂质，并对提取脂质的脂肪酸组成进行了表征。ILs 和甲醇的混合物成功地溶解了生物质，而使脂质不溶。用传统的 Bligh 和 Dyer 法从商用和培养的普通小球藻中分别提取了 10.6%和 11.1%的总脂质含量，而 [Bmim][CF(3)SO(3)]和甲醇的混合物分别提取了 12.5%和 19.0%的脂质。通过线性溶剂化能量关系的多参数回归表明，对于从藻类生物质中有效提取脂质，IL 的偶极矩/极化率和氢键酸度比氢键碱性更为重要。用 IL-甲醇混合物提取的脂质的脂肪酸组成表明，C16:0、C16:1、C18:2 和 C18:3 脂肪酸占主导地位。这表明从小球藻中提取的脂质可用作生物柴油生产的来源。
