Wei S M
Beijing Institute for Cancer Research.
Zhonghua Zhong Liu Za Zhi. 1989 May;11(3):162-4.
Spleen cells from Balb/c mice immunized in sequence with five human gastric cancer cell lines were fused with murine myeloma cell line SP2/0. Hybridomas 3F4, 3G9 and 3H11 secreting monoclonal antibodies (mAb) against gastric cancer were obtained through selective culture and screening. These mAb produced by the immunization procedure have good selectivity and high positive rate in reaction on gastric cancer. The positive rate of reaction on gastric cancer cells and tissues could reach 5/5 and 84.8-93.5%, respectively, whereas there was almost no positive reaction on normal cells and tissues As there was no correlation between the positive reaction of gastric cancer and their histopathologic typing, and the cross reaction of mAb with other tumors and fetal gastrointestinal tissues was quite high, the corresponding antigens of these mAb were considered as extensive oncofetal antigens.
将依次用五种人胃癌细胞系免疫的Balb/c小鼠的脾细胞与鼠骨髓瘤细胞系SP2/0进行融合。通过选择性培养和筛选，获得了分泌抗胃癌单克隆抗体（mAb）的杂交瘤3F4、3G9和3H11。通过免疫程序产生的这些单克隆抗体在对胃癌的反应中具有良好的选择性和高阳性率。对胃癌细胞和组织的反应阳性率分别可达5/5和84.8 - 93.5%，而对正常细胞和组织几乎没有阳性反应。由于胃癌的阳性反应与其组织病理学类型之间没有相关性，且单克隆抗体与其他肿瘤和胎儿胃肠道组织的交叉反应相当高，因此这些单克隆抗体的相应抗原被认为是广泛的癌胚抗原。
