Dong Z W
Zhonghua Zhong Liu Za Zhi. 1986 Jan;8(1):8-10.
Spleen cells of Balb/c mice, immunized with gastric cancer cell MGC 803, were fused with murine myeloma cell NS-1. After selective culture, screening and subcloning, a hybridoma PC1 which produced monoclonal antibody (McAb) against MGC 803 cells was obtained. McAb PC1 bound strongly with 3/4 gastric cancer and 1/2 hepatoma cell lines, weakly with another gastric cancer and 2/2 lung cancer cell lines, but did not bind with the autologous and allogenic lymphocytes, ABO red blood cells, human fetal lung fibroblasts and normal bone marrow cells. The binding capacity of McAb PC1 to MGC 803 decreased significantly due to the absorption by MGC 803 cells, but was not affected by lymphocytes and CEA. The corresponding antigen of McAb PC1 was expressed on the surface of MGC 803 cells. It may be a gastric cancer-associated antigen.
用胃癌细胞MGC 803免疫的Balb/c小鼠脾细胞与鼠骨髓瘤细胞NS-1融合。经过选择性培养、筛选和亚克隆，获得了一株产生抗MGC 803细胞单克隆抗体（McAb）的杂交瘤PC1。单克隆抗体PC1与3/4的胃癌细胞系和1/2的肝癌细胞系强烈结合，与另一胃癌细胞系和2/2的肺癌细胞系弱结合，但不与自体和同种异体淋巴细胞、ABO红细胞、人胚肺成纤维细胞及正常骨髓细胞结合。单克隆抗体PC1与MGC 803的结合能力因被MGC 803细胞吸收而显著降低，但不受淋巴细胞和癌胚抗原（CEA）影响。单克隆抗体PC1的相应抗原在MGC 803细胞表面表达。它可能是一种胃癌相关抗原。
