Badía J, Baldomà L, Aguilar J, Boronat A
Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, Spain.
FEMS Microbiol Lett. 1989 Dec;53(3):253-7. doi: 10.1016/0378-1097(89)90226-7.
The three structural genes rhaA, rhaB and rhaD, that specify the enzymes rhamnose isomerase, rhamnulose kinase and rhamnulose 1-phosphate aldolase respectively, have been cloned from Escherichia coli K-12. The precise location of the genes has been determined by gene complementation analysis and by enzymatic assays of strains transformed with recombinant plasmids containing different parts of the cloned region. The corresponding gene products have been studied by their expression in maxicells. Protein products of 47 kDa, 52-54 kDa and 32 kDa have been assigned to rhamnose isomerase, rhamnulose kinase and rhamnulose 1-phosphate aldolase respectively.
分别编码鼠李糖异构酶、鼠李糖酮激酶和鼠李糖酮-1-磷酸醛缩酶的三个结构基因rhaA、rhaB和rhaD已从大肠杆菌K-12中克隆出来。通过基因互补分析以及对用含有克隆区域不同部分的重组质粒转化的菌株进行酶活性测定，确定了这些基因的确切位置。通过在大细胞中的表达对相应的基因产物进行了研究。已分别将47 kDa、52 - 54 kDa和32 kDa的蛋白质产物归属于鼠李糖异构酶、鼠李糖酮激酶和鼠李糖酮-1-磷酸醛缩酶。
