Makni M, Marrakchi M, Prud'homme N
Genet Res. 1989 Oct;54(2):127-35. doi: 10.1017/s0016672300028494.
In Drosophila melanogaster up to two thirds of the rDNA genes contain insertion sequences of two types in the 28S coding region. Comparison of the ribosomal insertion transcripts in the wild type and in two bobbed mutants reared at two temperatures showed that the level of type I transcripts is dependent on both the number of genes with type I insertions in the bobbed loci and the intensity of bobbed phenotype. Importantly, a long transcript of 8.7 kb hybridized to the ribosomal probe, the INS I probe and also to the restriction fragment of the rDNA downstream of the point of insertion was found in one bobbed mutant. This result and also those from sandwich hybridization indicate that some interrupted ribosomal genes are functional.
在黑腹果蝇中，多达三分之二的核糖体DNA（rDNA）基因在28S编码区域含有两种类型的插入序列。对野生型以及在两种温度下饲养的两个截毛突变体中的核糖体插入转录本进行比较，结果表明I型转录本的水平既取决于截毛位点中含有I型插入的基因数量，也取决于截毛表型的强度。重要的是，在一个截毛突变体中发现了一个8.7 kb的长转录本，它与核糖体探针、INS I探针杂交，并且还与插入点下游rDNA的限制性片段杂交。这一结果以及夹心杂交的结果表明，一些中断的核糖体基因是有功能的。
