家蚕28S核糖体基因含有与黑腹果蝇I型和II型元件相似的插入元件。
Bombyx mori 28S ribosomal genes contain insertion elements similar to the Type I and II elements of Drosophila melanogaster.
作者信息
Eickbush T H, Robins B
出版信息
EMBO J. 1985 Sep;4(9):2281-5. doi: 10.1002/j.1460-2075.1985.tb03927.x.
Abstract
We have examined the 28S ribosomal genes of the silkmoth, Bombyx mori, for the presence of insertion sequences. Two types of insertion sequences were found, each approximately 5 kb in length, which do not share sequence homology. Comparison of the nucleotide sequences of the junction regions with the uninserted gene reveals that one type of insertion has resulted in a 14 bp duplication of the 28S coding region at the insertion site. The location of this insertion and the 14 bp duplication are identical to that found in the Type I ribosomal insertion element of Drosophila melanogaster. The second type of insertion element is located at a site corresponding to approximately 75 bp upstream of the first type. The location of this insertion, the variability detected at its 5' junction, and a short region of sequence homology at its 3' junction suggest that it is related to the Type II element of D. melanogaster. This is the first example of a Type II-like rDNA insertion outside of sibling species of D. melanogaster, and the first example of a Type I-like rDNA insertion outside of the higher Diptera.
摘要
我们检测了家蚕（Bombyx mori）的28S核糖体基因，以寻找插入序列的存在。发现了两种类型的插入序列，每种长度约为5 kb，它们不具有序列同源性。将插入区域的核苷酸序列与未插入基因进行比较，发现一种类型的插入导致了28S编码区域在插入位点处有14 bp的重复。该插入的位置以及14 bp的重复与在黑腹果蝇（Drosophila melanogaster）的I型核糖体插入元件中发现的情况相同。第二种类型的插入元件位于与第一种类型上游约75 bp相对应的位置。该插入的位置、在其5'连接处检测到的变异性以及在其3'连接处的短序列同源区域表明它与黑腹果蝇的II型元件有关。这是在黑腹果蝇的近缘种之外首次发现类似II型的rDNA插入的例子，也是在双翅目高级类群之外首次发现类似I型的rDNA插入的例子。
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