Dai Xiaofeng, Shi Xiaochong, Gao Xin, Liang Jing, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1122-1126. doi: 10.1099/ijs.0.000066. Epub 2015 Jan 14.
A Gram-stain-negative, facultatively anaerobic, chemoheterotrophic, moderately halophilic, exopolysaccharide (EPS)-producing, cream, non-motile and rod-shaped bacterium, designated strain ZH114(T), was isolated from deep water of the South China Sea, and was subjected to a polyphasic taxonomic study. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that this strain belongs to the genus Salipiger with the highest sequence similarity to Salipiger mucescens LMG 22090(T) (96.83%), followed by Pseudodonghicola xiamenensis LMG 24574(T) (96.12%). Growth occurred at 4-37 °C (optimum 32 °C), pH 6.0-10.0 (optimum pH 9.0-10.0) and in the presence of 0-19% NaCl (w/v) (optimum 6%, w/v). It did not produce poly-β-hydroxyalkanoate granules or bacteriochlorophyll a. Acid was produced from glycerol, erythrose, ribose, D-xylose, galactose, glucose, fructose, mannitol, cellobiose, maltose, lactose, melibiose, turanose, D-lyxose, D-tagatose, D-fucose, D-arabitol and L-arabitol after inoculating for 24 h and weakly positive results were also detected after 48 h in API 50CH strips with D-arabinose, L-arabinose, L-xylose, adonitol, mannose, aesculin, salicin, sucrose, mycose and L-fucose. The predominant fatty acids were C(18 : 1)ω7c and/or C(18 : 1)ω6c, C(16 : 0), C(18 : 0) and 11-methyl C(18 : 1)ω7c. The major polar lipids of ZH114(T) were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The major respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content of strain ZH114(T) was 63.8 mol%. Based on this phenotypic, chemotaxonomic and phylogenetic analysis, strain ZH114(T) should be classified as a representative of a novel species of the genus Salipiger , for which the name Salipiger nanhaiensis sp. nov. is proposed. The type strain is ZH114(T) ( = JCM 19383(T) = KCTC 32468(T)).
从中国南海深水中分离出一株革兰氏阴性、兼性厌氧、化能异养、中度嗜盐、产胞外多糖（EPS）、呈奶油色、无运动性的杆状细菌，命名为菌株ZH114(T)，并对其进行了多相分类研究。基于16S rRNA基因序列的系统发育分析表明，该菌株属于盐栖菌属，与黏液盐栖菌LMG 22090(T)序列相似性最高（96.83%），其次是厦门假东海菌LMG 24574(T)（96.12%）。该菌在4-37 °C（最适温度32 °C）、pH 6.0-10.0（最适pH 9.0-10.0）以及0-19% NaCl（w/v）（最适6%，w/v）条件下生长。它不产生聚-β-羟基链烷酸酯颗粒或细菌叶绿素a。接种24 h后，该菌可从甘油、赤藓糖、核糖、D-木糖、半乳糖、葡萄糖、果糖、甘露醇、纤维二糖、麦芽糖、乳糖、蜜二糖、松二糖、D-来苏糖、D-塔格糖、D-岩藻糖、D-阿拉伯糖醇和L-阿拉伯糖醇产酸，在API 50CH条中接种48 h后，对D-阿拉伯糖、L-阿拉伯糖、L-木糖、阿东糖醇、甘露糖、七叶苷、水杨苷、蔗糖、蜜三糖和L-岩藻糖也检测到弱阳性结果。主要脂肪酸为C(18 : 1)ω7c和/或C(18 : 1)ω6c、C(16 : 0)、C(18 : 0)和11-甲基C(18 : 1)ω7c。ZH114(T)的主要极性脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺和两种未鉴定的脂类。主要呼吸醌为泛醌Q-10。菌株ZH114(T)的基因组DNA G+C含量为63.8 mol%。基于此表型、化学分类和系统发育分析，菌株ZH114(T)应被归类为盐栖菌属一个新物种的代表，为此提出盐栖南海菌新种Salipiger nanhaiensis sp. nov.。模式菌株为ZH114(T)（=JCM 19383(T)=KCTC 32468(T)）。
