Ocean College, Zhejiang University, No. 1 Zheda Rd., Zhoushan 316000, PR China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2016 Aug;66(8):3029-3033. doi: 10.1099/ijsem.0.001140. Epub 2016 May 6.
A novel, Gram-stain-negative, motile, facultatively anaerobic bacterium, designated strain NF48T, was isolated from surface seawater around Zhoushan Islands. Cells were rod-shaped (1.1-3.9×0.5-0.9 µm). Strain NF48T was able to grow at 10-40 °C (optimum 30 °C), at pH 5.5-9.0 (optimum 6.5-8.0) and with 0.5-7.0 % (w/v) NaCl (optimum 2.0 %). The genomic DNA G+C content was 65.5 mol%. Chemotaxonomic analysis showed that the main isoprenoid quinone was Q-10 and the major fatty acids were C18 : 1ω7c and C16 : 0. The major polar lipids of strain NF48T were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NF48T belonged to the genus Seohaeicola with similarity values of 95.5-97.2 % to members of this genus, and was most closely related to Seohaeicola nanhaiensis SS011A0-7#2-2T (97.2 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain NF48T is suggested to represent a novel species of the genus Seohaeicola, for which the name Seohaeicola zhoushanensis sp. nov. is proposed. The type strain is NF48T (=MCCC 1K01157T=KCTC 42650T).
一株新的、革兰氏阴性、运动性、兼性厌氧细菌，被命名为 NF48T 菌株，从舟山群岛周围的表层海水中分离得到。细胞呈杆状（1.1-3.9×0.5-0.9μm）。NF48T 菌株能够在 10-40°C（最适温度 30°C）、pH5.5-9.0（最适 pH6.5-8.0）和 0.5-7.0%（w/v）NaCl（最适 2.0%）的条件下生长。基因组 DNA G+C 含量为 65.5mol%。化学分类分析表明，主要的异戊二烯醌是 Q-10，主要的脂肪酸是 C18:1ω7c 和 C16:0。NF48T 菌株的主要极性脂是磷脂酰胆碱、磷脂乙醇胺和磷脂酰甘油。基于 16S rRNA 基因序列的系统发育分析表明，NF48T 菌株属于 Seohaeicola 属，与该属的其他成员的相似性值为 95.5-97.2%，与 Seohaeicola nanhaiensis SS011A0-7#2-2T（97.2%）最为密切相关。基于表型、化学分类和系统发育特征，NF48T 菌株被建议代表 Seohaeicola 属的一个新种，命名为 Seohaeicola zhoushanensis sp. nov.。模式菌株为 NF48T（=MCCC 1K01157T=KCTC 42650T）。
