Department of Physical Chemistry, Chemistry Institute, São Paulo State University (UNESP, Universidade Estadual Paulista), Nanobionics Research Group, 55 Prof. Francisco Degni Street, 14800-060 Araraquara, São Paulo, Brazil(2).
Department of Physical Chemistry, Chemistry Institute, São Paulo State University (UNESP, Universidade Estadual Paulista), Nanobionics Research Group, 55 Prof. Francisco Degni Street, 14800-060 Araraquara, São Paulo, Brazil(2).
Biosens Bioelectron. 2015 Jun 15;68:281-287. doi: 10.1016/j.bios.2014.12.059. Epub 2014 Dec 31.
Early detection assays play a key role in the successful treatment of most diseases. Redox capacitive biosensors were recently introduced as a potential electroanalytical assay platform for point-of-care applications but alternative surfaces (besides a mixed layer containing ferrocene and antibody receptive component) for recruiting important clinical biomarkers are still needed. Aiming to develop alternative receptive surfaces for this novel electrochemical biosensing platform, we synthesized a ferrocene redox-tagged peptide capable of self-assembly into metallic interfaces, a potentially useful biological surface functionalization for bedside diagnostic assays. As a proof of concept we used C-reactive protein (CRP), as a model biomarker, and compared the obtained results to those of previously reported capacitive assays. The redox-tagged peptide approach shows a limit of detection of 0.8 nmol L(-1) (same as 94 ng mL(-1)) and a linear range (R(2)∼98%) with the logarithm of the concentration of the analyte comprising 0.5-10.0 nmol L(-1), within a clinical relevant range for CRP.
早期检测在大多数疾病的成功治疗中起着关键作用。氧化还原电容生物传感器最近被引入作为一种潜在的用于即时检测应用的电化学生物分析检测平台，但仍需要替代表面（除了包含二茂铁和抗体接受性成分的混合层）来招募重要的临床生物标志物。为了为这个新型电化学生物传感平台开发替代接受表面，我们合成了一种能够自组装成金属界面的二茂铁氧化还原标记肽，这是一种用于床边诊断检测的潜在有用的生物表面功能化方法。作为概念验证，我们使用 C 反应蛋白（CRP）作为模型生物标志物，并将获得的结果与之前报道的电容检测进行了比较。氧化还原标记肽方法的检测限为 0.8 nmol L(-1)（与 94 ng mL(-1)相同），线性范围（R(2)∼98%）与分析物浓度的对数在 0.5-10.0 nmol L(-1)之间，这在 CRP 的临床相关范围内。
