Instituto de Química, Universidade Estadual Paulista, CP 355, 14800-900 Araraquara, São Paulo, Brazil.
Biosens Bioelectron. 2013 Dec 15;50:437-40. doi: 10.1016/j.bios.2013.06.043. Epub 2013 Jul 2.
A surface confined redox group contributes to an interfacial charging (quantifiable by redox capacitance) that can be sensitively probed by impedance derived capacitance spectroscopy. In generating mixed molecular films comprising such redox groups, together with specific recognition elements (here antibodies), this charging signal is able to sensitively transduce the recognition and binding of specific analytes. This novel transduction method, exemplified here with C-reactive protein, an important biomarker of cardiac status and general trauma, is equally applicable to any suitably prepared interfacial combination of redox reporter and receptor. The assays are label free, ultrasensitive, highly specific and accompanied by a good linear range.
一个表面受限的氧化还原基团有助于界面电荷（可通过氧化还原电容来量化），而界面电荷可以通过阻抗衍生电容光谱法来灵敏地探测。在生成包含这种氧化还原基团的混合分子膜时，同时结合特定的识别元件（这里是抗体），这种电荷信号能够灵敏地转导特定分析物的识别和结合。这种新的转导方法，这里以 C-反应蛋白（一种心脏状态和一般创伤的重要生物标志物）为例，同样适用于任何经过适当制备的氧化还原报告基团和受体之间的界面组合。这些检测方法是无标记的、超灵敏的、高度特异的，并且伴随着良好的线性范围。
