Yan Q, Johnson E M
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
J Comp Neurol. 1989 Dec 22;290(4):585-98. doi: 10.1002/cne.902900411.
The expression of nerve growth factor (NGF) receptor in adult rat brain was studied by immunohistochemistry with a specific anti-rat NGF receptor monoclonal antibody, 192-IgG. Intense NGF receptor immunoreactivity (NGFRI) was found in structures known to be NGF responsive, including forebrain cholinergic neurons in medial septum, diagonal band of Broca, and basal nucleus of Meynert; central processes of neural-crest-derived sensory ganglion neurons and their innervated nucleus also contained such immunoreactivity. Distinct NGFRI staining was also found in many brain areas and cell types not known to be NGF responsive, including some hypothalamic regions, circumventricular organs, some areas related to the optic system, olfactory glomeruli, ependymal and subependymal cells in some locations, mesencephalic nucleus of the trigeminal nerve, cerebellar molecular layer, central linear nucleus, solitary tract and its nucleus, and inferior olive. The NGFRI in the circumventricular organs was further studied by in vivo labeling of 125I-ligands. Intravenously injected 125I-NGF, but not 125I-cytochrome c, was specifically accumulated in the area postrema. Biochemical study of the NGF receptor showed a major band of molecular weight of approximately 90 KDa in the area postrema, choroid plexus, median eminence, and medial septum with the relative content consistent with that seen by immunohistochemistry. No evidence of a truncated NGF receptor was observed. The results of this study suggest that NGF and its receptor have broader roles in adult mammalian brain than previously thought.
采用特异性抗大鼠神经生长因子（NGF）受体单克隆抗体192-IgG，通过免疫组织化学方法研究了成年大鼠脑中NGF受体的表达情况。在已知对NGF有反应的结构中发现了强烈的NGF受体免疫反应性（NGFRI），包括内侧隔、布罗卡斜带和迈内特基底核中的前脑胆碱能神经元；神经嵴衍生的感觉神经节神经元的中枢突及其支配的核也含有这种免疫反应性。在许多未知对NGF有反应的脑区和细胞类型中也发现了明显的NGFRI染色，包括一些下丘脑区域、室周器官、一些与视觉系统相关的区域、嗅小球、某些部位的室管膜和室管膜下细胞、三叉神经中脑核、小脑分子层、中央线性核、孤束及其核以及下橄榄核。通过125I配体的体内标记进一步研究了室周器官中的NGFRI。静脉注射的125I-NGF，而不是125I-细胞色素c，特异性地积聚在最后区。对NGF受体的生化研究表明，在最后区、脉络丛、正中隆起和内侧隔中存在一条分子量约为90 kDa的主要条带，其相对含量与免疫组织化学所见一致。未观察到截短的NGF受体的证据。本研究结果表明，NGF及其受体在成年哺乳动物脑中的作用比以前认为的更为广泛。
