Bruckner-Tuderman L, Pfaltz M, Schnyder U W
Department of Dermatology, University Hospital Zürich, Switzerland.
J Invest Dermatol. 1991 May;96(5):729-34. doi: 10.1111/1523-1747.ep12470965.
The cell clusters of the human adnexal tumor, cylindroma, are surrounded by an unusual, thick, unorganized basement membrane-like zone in vivo. Ultrastructural analysis of this electron-dense zone revealed recesses that contained numerous anchoring fibrils. Indirect immunofluorescence staining with antibodies to collagen VII, the major structural component of the anchoring fibrils, showed strong fluorescence of this zone. Collagen VII could be isolated from tumor tissue by limited pepsin digestion or by extraction with chaotropic agents. Cylindroma cells were cultured from the tumor and passaged several times. The cells exhibited a strong expression of collagen VII in vitro, when assayed with immunofluorescence staining or immunoblotting of culture extracts. These results suggest that cylindroma can be used as an abundant source to produce collagen VII in vitro to study the biosynthesis and regulation of collagen VII and the formation of the anchoring fibrils, as other culture systems have failed to produce ample amounts of this anchoring fibril protein.
在人体内，人附属器肿瘤圆柱瘤的细胞团簇被一个不寻常的、厚厚的、无组织的类似基底膜的区域所包围。对这个电子致密区的超微结构分析显示，其中含有许多锚定纤维的凹陷。用针对锚定纤维主要结构成分Ⅶ型胶原的抗体进行间接免疫荧光染色，结果显示该区域有强烈荧光。通过有限的胃蛋白酶消化或用离液剂提取，可从肿瘤组织中分离出Ⅶ型胶原。从肿瘤中培养出圆柱瘤细胞并传代培养了几次。当用免疫荧光染色或对培养提取物进行免疫印迹分析时，这些细胞在体外表现出强烈的Ⅶ型胶原表达。这些结果表明，圆柱瘤可作为体外生产Ⅶ型胶原的丰富来源，用于研究Ⅶ型胶原的生物合成、调控以及锚定纤维的形成，因为其他培养系统未能产生足够量的这种锚定纤维蛋白。
