Larrieu Antoine, Champion Antony, Legrand Jonathan, Lavenus Julien, Mast David, Brunoud Géraldine, Oh Jaesung, Guyomarc'h Soazig, Pizot Maxime, Farmer Edward E, Turnbull Colin, Vernoux Teva, Bennett Malcolm J, Laplaze Laurent
1] Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France [2] Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, UK.
1] Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France [2] Laboratoire mixte international Adaptation des Plantes et microorganismes associés aux Stress Environnementaux, CP 18534 Dakar, Senegal.
Nat Commun. 2015 Jan 16;6:6043. doi: 10.1038/ncomms7043.
Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.
茉莉酸（JA）的活性形式是协调植物对胁迫反应的核心信号，但缺乏分析其时空分布的工具。在此，我们描述了一种名为Jas9-VENUS的JA感知生物传感器，它能够以高时空灵敏度定量分析胁迫响应中JA分布的动态变化。我们发现Jas9-VENUS的丰度取决于生物活性JA异构体、COI1共受体、功能性Jas基序和蛋白酶体活性。我们展示了Jas9-VENUS在细胞水平上定量和动态分析植物对JA反应的实用性。这包括使用Jas9-VENUS来确定受伤时子叶到根的JA信号速度，揭示了根中JA活性的两个不同阶段。我们的结果证明了开发如Jas9-VENUS这样的定量传感器的价值，以提供关于植物非生物和生物胁迫响应中激素分布的高分辨率时空数据。
