Zhu Xinyue, Wang Jianxi, Zhang Jianjian, Chen Zhenjie, Zhang Haixia, Zhang Xiaoyu
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
Sensors (Basel). 2015 Jan 14;15(1):1611-22. doi: 10.3390/s150101611.
A reaction-based two-photon (TP) ratiometric fluorescence probe Z2 has been developed and successfully applied to detect and image fluoride ion in living cells and tissues. The Z2 probe was designed designed to utilize an ICT mechanism between n-butylnaphthalimide as a fluorophore and tert-butyldiphenylsilane (TBDPS) as a response group. Upon addition of fluoride ion, the Si-O bond in the Z2 would be cleaved, and then a stronger electron-donating group was released. The fluorescent changes at 450 and 540 nm, respectively, made it possible to achieve ratiometric fluorescence detection. The results indicated that the Z2 could ratiometrically detect and image fluoride ion in living cells and tissues in a depth of 250 μm by two-photon microscopy (TPM).
一种基于反应的双光子（TP）比率荧光探针Z2已被开发出来，并成功应用于活细胞和组织中氟离子的检测与成像。Z2探针的设计利用了作为荧光团的正丁基萘二甲酰亚胺与作为响应基团的叔丁基二苯基硅烷（TBDPS）之间的ICT机制。加入氟离子后，Z2中的Si-O键会断裂，然后释放出一个更强的供电子基团。分别在450和540 nm处的荧光变化使得实现比率荧光检测成为可能。结果表明，Z2可以通过双光子显微镜（TPM）在深度为250μm的活细胞和组织中对氟离子进行比率检测和成像。
