宋氏叶下珠的细胞毒性和自然杀伤细胞刺激成分。
Cytotoxic and natural killer cell stimulatory constituents of Phyllanthus songboiensis.
作者信息
Ren Yulin, Yuan Chunhua, Deng Youcai, Kanagasabai Ragu, Ninh Tran Ngoc, Tu Vuong Tan, Chai Hee-Byung, Soejarto Djaja D, Fuchs James R, Yalowich Jack C, Yu Jianhua, Kinghorn A Douglas
机构信息
Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Campus Chemical Instrument Center, The Ohio State University, Columbus, OH 43210, USA.
出版信息
Phytochemistry. 2015 Mar;111:132-40. doi: 10.1016/j.phytochem.2014.12.014. Epub 2015 Jan 14.
A dichapetalin-type triterpenoid and a dibenzylbutyrolactone-type lignan, together with five known lignans, a known aromatic diterpenoid, and a known acylated phytosterol, were isolated from the aerial parts of Phyllanthus songboiensis, collected in Vietnam. Their structures were determined by interpretation of the spectroscopic data, and the inhibitory activity toward HT-29 human colon cancer cells of all isolates was evaluated by a cytotoxicity assay. The known arylnaphthalene lignan, (+)-acutissimalignan A, was highly cytotoxic toward HT-29 cells, with an IC50 value of 19 nM, but this compound was inactive as a DNA topoisomerase IIα (topo IIα) poison. The known phytosterol, (-)-β-sitosterol-3-O-β-D-(6-O-palmitoyl)glucopyranoside, was found to stimulate natural killer (NK) cells at a concentration of 10μM in the presence of interleukin 12 (IL-12).
从采自越南的宋氏叶下珠地上部分分离得到了一种二萜内酯型三萜类化合物和一种二苄基丁内酯型木脂素，以及五种已知木脂素、一种已知芳香二萜类化合物和一种已知酰化植物甾醇。通过光谱数据解析确定了它们的结构，并通过细胞毒性试验评估了所有分离物对HT-29人结肠癌细胞的抑制活性。已知的芳基萘木脂素（+）-急尖叶下珠脂素A对HT-29细胞具有高度细胞毒性，IC50值为19 nM，但该化合物作为DNA拓扑异构酶IIα（拓扑异构酶IIα）毒药无活性。发现已知植物甾醇（-）-β-谷甾醇-3-O-β-D-（6-O-棕榈酰基）吡喃葡萄糖苷在白细胞介素12（IL-12）存在下，浓度为10μM时可刺激自然杀伤（NK）细胞。
Zotero 插件