State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, P. R. China.
Small. 2015 May 13;11(18):2200-8. doi: 10.1002/smll.201402531. Epub 2015 Jan 19.
Circulating tumor cells (CTCs) captured from blood fluid represent recurrent cancers and metastatic lesions to monitor the situation of cancers. We develop surface-enhanced Raman scattering (SERS)-coding microsphere suspension chip as a new strategy for fast and efficient capture, recovery, and detection of targeting cancer cells. Using HeLa cells as model CTCs, we first utilize folate as a recognition molecule to be immobilized in magnetic composite microspheres for capturing HeLa cells and attaining high capturing efficacy (up to 95%). After capturing cells, the composite microsphere, which utilizes a disulfide bond as crosslinker in the polymer shell and as a spacer for linking folate, can recycle 90% cells within 20 min eluted by glutathion solution. Taking advantage of the SERS with fingerprint features, we characterize captured/recovered cells with the unique signal of report-molecule 4-aminothiophenol through introducing the SERS-coding microsphere suspension chip to CTCs. Finally, the exploratory experiment of sieving cells shows that the magnetic composite microspheres can selectively capture the HeLa cells from samples of mixed cells, indicating that these magnetic composite microspheres have potential in real blood samples for capturing CTCs.
从血液流体中捕获的循环肿瘤细胞 (CTCs) 代表复发癌症和转移病变，以监测癌症的情况。我们开发了表面增强拉曼散射 (SERS)-编码微球悬浮芯片作为一种新的策略，用于快速有效地捕获、回收和检测靶向癌细胞。我们首先使用叶酸作为识别分子，将其固定在磁性复合微球中，用于捕获 HeLa 细胞，并获得了高达 95%的高捕获效率。捕获细胞后，利用聚合物壳中的二硫键作为交联剂，以及作为连接叶酸的间隔物的二硫键，复合微球可以在 20 分钟内通过谷胱甘肽溶液洗脱回收 90%的细胞。利用具有指纹特征的 SERS，我们通过引入 SERS 编码微球悬浮芯片对捕获/回收的细胞进行了特征化，这些细胞具有报告分子 4-巯基苯硼酸的独特信号。最后，通过细胞筛选的探索性实验表明，磁性复合微球可以从混合细胞的样本中选择性地捕获 HeLa 细胞，这表明这些磁性复合微球在实际血液样本中具有捕获 CTCs 的潜力。
