Bafrani Hassan Hassani, Parsa Yekta, Yadollah-Damavandi Soheila, Jangholi Ehsan, Ashkani-Esfahani Soheil, Gharehbeglou Mohammad
Kashan Anatomical Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Students' Research Committee, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
N Am J Med Sci. 2014 Dec;6(12):638-42. doi: 10.4103/1947-2714.147981.
Nephrolithiasis is of the most prevalent urinary tract disease. It seems worthwhile to replace the conventional treatments with more beneficial and safer agents, particularly herbal medicines which are receiving an increasing interest nowadays.
In this study, we investigated the protective and curative effects of Achillea millefolium L. on ethylene glycol (EG)-induced nephrolithiasis in rats.
The extract of A. millefolium was prepared by soxhlet method. Forty male Wistar rats were randomly divided into five groups (N = 8) as follows. The negative control (group A) received tap drinking water. Rats in sham (positive control group B), curative (group C and D), and preventive (group E) groups all received 1% EG in drinking water according to the experimental protocol for 30 days. In the curative groups, dosages of 200 and 400 mg/kg body weight (BW) of A. millefolium extract were administered orally from day 15 to the end of the experiment, group C and D, respectively. Group E received 200 mg/kg A. millefolium extract from the 1(st) day throughout the experiment. Urinary oxalate and citrate concentrations were measured by spectrophotometer on the first and 30(th) days. On day 31, the kidneys were removed and examined histopathologically for counting the calcium oxalate (CaOx) deposits in 50 microscopic fields.
In the curative and preventive groups, administration of A. millefolium extract showed significant reduction in urinary oxalate concentration (P < 0.05). Also, urinary citrate concentration was significantly increased in group C, D, and E. The CaOx deposits significantly decreased in group C to E compared with the group B.
According to our results, A. millefolium extract had preventive and curative effects on EG-induced renal calculi.
肾结石是最常见的泌尿系统疾病之一。用更有益且更安全的药物替代传统治疗方法似乎是值得的，尤其是如今越来越受关注的草药。
在本研究中，我们调查了蓍草对乙二醇（EG）诱导的大鼠肾结石的保护和治疗作用。
采用索氏提取法制备蓍草提取物。40只雄性Wistar大鼠随机分为五组（每组n = 8），分组如下。阴性对照组（A组）饮用自来水。假手术组（阳性对照组B）、治疗组（C组和D组）以及预防组（E组）的大鼠均按照实验方案饮用含1% EG的水30天。在治疗组中，从第15天至实验结束，C组和D组分别口服剂量为200和400 mg/kg体重（BW）的蓍草提取物。E组从第1天至整个实验过程中均接受200 mg/kg的蓍草提取物。在第1天和第30天用分光光度计测量尿草酸和柠檬酸盐浓度。在第31天，取出肾脏并进行组织病理学检查，以计数50个显微镜视野中的草酸钙（CaOx）沉积物。
在治疗组和预防组中，蓍草提取物的给药使尿草酸浓度显著降低（P < 0.05）。此外，C组、D组和E组的尿柠檬酸盐浓度显著升高。与B组相比，C组至E组的CaOx沉积物显著减少。
根据我们的结果，蓍草提取物对EG诱导的肾结石具有预防和治疗作用。
