Thoe S Y, Sam C K, Cheng H M, Prasad U
Institute of Advanced Studies, University of Malaya, Kuala Lumpur, Malaysia.
J Med Virol. 1989 Dec;29(4):311-4. doi: 10.1002/jmv.1890290417.
Serum antibodies against Epstein-Barr virus (EBV)-determined antigens have traditionally been titrated by the indirect immunofluorescence (IIF) technique. The avidin-biotin complex (ABC) immunocytochemical technique was used to determine the serum levels of IgA against EBV viral capsid antigen (IgA/VCA) and IgA against EBV early antigen (IgA/EA) in sera of 106 nasopharyngeal carcinoma (NPC) patients prior to treatment and 100 normal individuals. The sensitivity of the ABC technique is enhanced by an amplification of the antigen-antibody reaction, which involves the binding of the enzyme-linked ABC to the second biotinylated antibody. There was a good correlation (r = 0.9988) between ABC and IIF-determined IgA/VCA-positive titres, with the ABC technique being more sensitive than IIF in the detection of IgA/VCA in NPC sera: 94% (99/106) and 76% (80/106), respectively. The frequency of IgA/EA reactivity in NPC sera was also markedly increased by immunodetection with the ABC technique as compared with IIF technique: 63% (69/106) and 28% (30/106) respectively. Both the immunocytochemical techniques were equally specific in discriminating between elevated serum titres of IgA/VCA and IgA/EA in NPC sera from normal human sera.
传统上，针对爱泼斯坦-巴尔病毒（EBV）特定抗原的血清抗体是通过间接免疫荧光（IIF）技术进行滴定的。采用抗生物素蛋白-生物素复合物（ABC）免疫细胞化学技术，检测了106例鼻咽癌（NPC）患者治疗前血清及100例正常人血清中抗EBV病毒衣壳抗原的IgA（IgA/VCA）和抗EBV早期抗原的IgA（IgA/EA）水平。ABC技术通过放大抗原-抗体反应来提高灵敏度，该反应涉及酶联ABC与第二种生物素化抗体的结合。ABC法与IIF法测定的IgA/VCA阳性滴度之间具有良好的相关性（r = 0.9988），在检测NPC血清中的IgA/VCA时，ABC技术比IIF更敏感：分别为94%（99/106）和76%（80/106）。与IIF技术相比，ABC技术免疫检测时NPC血清中IgA/EA反应性的频率也显著增加：分别为63%（69/106）和28%（30/106）。在区分NPC血清和正常人血清中IgA/VCA和IgA/EA升高的血清滴度方面，两种免疫细胞化学技术的特异性相同。
