Canisso Igor F, Ball Barry A, Scoggin Kirsten E, Squires Edward L, Williams Neil M, Troedsson Mats H
Reproduction Laboratory, Maxwell Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA.
Reproduction Laboratory, Maxwell Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA.
Anim Reprod Sci. 2015 Mar;154:48-55. doi: 10.1016/j.anireprosci.2014.12.019. Epub 2015 Jan 9.
The objectives of this study were to: (i) determine alpha-fetoprotein (AFP) concentrations in fetal fluids (FF), and (ii) compare plasma concentrations of AFP in mares with placentitis (n=17) and gestationally age-matched control mares (n=17). Fetal fluid sampling (FFS, n=7/group) was performed at 0, 5 and 12 days post inoculation (DPI) or until abortion. Plasma was harvested daily for 12 days or until abortion. Placentitis was induced via intracervical inoculation of Streptococcus equi ssp. zooepidemicus. Proteins present in the FF were resolved by 1D-SDS-PAGE, and immunoblotting was used to detect the presence of AFP in fetal fluids. Concentrations of AFP in FF and plasma were determined with a chemiluminescence immunoassay. Mixed models for DPI, and for days from abortion (DFA) were used to analyze plasma concentrations of AFP. A protein band ∼68kDa consistent with the AFP size was present in all samples of fetal fluids examined. Immunoblotting for AFP revealed a single protein band (∼68kDa) in all samples. Concentrations of AFP in FF appeared higher than those in maternal plasma. There were effects of time (DPI p<0.0001; DFA p=0.0002) and time-by-group interactions (DPIGroup p<0.06; GroupDFA p<0.001). This study confirmed that AFP is present in the FF of mares during the third trimester of pregnancy. Experimentally induced placentitis was associated with an elevation in maternal plasma concentrations of AFP.
（i）测定胎儿体液（FF）中的甲胎蛋白（AFP）浓度，以及（ii）比较患有胎盘炎的母马（n = 17）和妊娠年龄匹配的对照母马（n = 17）的血浆AFP浓度。在接种后0、5和12天（DPI）或直至流产时进行胎儿体液采样（FFS，每组n = 7）。每天采集血浆，持续12天或直至流产。通过子宫颈接种马链球菌兽疫亚种诱导胎盘炎。FF中存在的蛋白质通过一维SDS-PAGE进行分离，免疫印迹法用于检测胎儿体液中AFP的存在。使用化学发光免疫分析法测定FF和血浆中AFP的浓度。采用DPI以及流产天数（DFA）的混合模型来分析血浆AFP浓度。在所检测的所有胎儿体液样本中均出现了一条与AFP大小一致的约68kDa的蛋白条带。AFP免疫印迹显示所有样本中均有一条单一蛋白条带（约68kDa）。FF中AFP的浓度似乎高于母马血浆中的浓度。存在时间效应（DPI p<0.0001；DFA p = 0.0002）以及时间×组间交互作用（DPI组p<0.06；组DFA p<0.001）。本研究证实，妊娠晚期母马的FF中存在AFP。实验性诱导的胎盘炎与母马血浆中AFP浓度升高有关。
