Canisso Igor F, Ball Barry A, Cray Carolyn, Williams Neil M, Scoggin Kirsten E, Davolli Gabriel M, Squires Edward L, Troedsson Mats H
Reproduction Laboratory, The Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
Am J Reprod Immunol. 2014 Oct;72(4):376-85. doi: 10.1111/aji.12278. Epub 2014 Jun 11.
Currently, placentitis, an important cause of late pregnancy loss in mares, is diagnosed by clinical signs and ultrasonography. Acute phase proteins (APP) are mainly produced and secreted by the liver in response to acute inflammatory stimuli. We hypothesized that APP are increased in mares with placentitis.
Concentrations of serum amyloid A (SAA), haptoglobin (Hp), fibrinogen (Fb), and white blood cell counts (WBC) were determined in plasma of mares with experimentally induced placentitis and gestationally age-matched control mares. Placentitis was induced via intracervical inoculation of Streptococcus equi subspecies zooepidemicus, a common isolate from clinical cases of bacterial placentitis. Concentrations of SAA and Hp were also determined in the 10 days pre-partum in normal mares.
Mares with placentitis aborted within 5-25 days after inoculation. Concentrations of SAA and Hp rapidly increased subsequent to experimental induction of placentitis and remained increased until abortion. Neither Fb nor WBC appeared to be useful markers for placentitis. Parturition did not trigger increase in either SAA or Hp in normal foaling mares.
目前，胎盘炎是母马妊娠晚期流产的一个重要原因，通过临床症状和超声检查来诊断。急性期蛋白（APP）主要由肝脏在急性炎症刺激下产生和分泌。我们假设患有胎盘炎的母马体内APP会增加。
测定了实验性诱导胎盘炎的母马和妊娠年龄匹配的对照母马血浆中血清淀粉样蛋白A（SAA）、触珠蛋白（Hp）、纤维蛋白原（Fb）的浓度以及白细胞计数（WBC）。通过宫颈内接种马链球菌兽疫亚种诱导胎盘炎，该菌是细菌性胎盘炎临床病例中常见的分离菌株。还测定了正常母马产前10天内SAA和Hp的浓度。
患有胎盘炎的母马在接种后5 - 25天内流产。实验诱导胎盘炎后，SAA和Hp的浓度迅速升高，并一直保持升高直至流产。Fb和WBC似乎都不是胎盘炎的有用标志物。正常分娩的母马分娩时SAA和Hp均未升高。
