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适用于所有RNA类型的链特异性全转录组RNA测序

Stranded Whole Transcriptome RNA-Seq for All RNA Types.

作者信息

Miller David F B, Yan Pearlly X, Fang Fang, Buechlein Aaron, Ford James B, Tang Haixu, Huang Tim H, Burow Matthew E, Liu Yunlong, Rusch Douglas B, Nephew Kenneth P

机构信息

Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana.

Department of Internal Medicine, OSUCCC-Illumina Core, Columbus, Ohio.

出版信息

Curr Protoc Hum Genet. 2015 Jan 20;84:11.14.1-11.14.23. doi: 10.1002/0471142905.hg1114s84.

DOI:10.1002/0471142905.hg1114s84
PMID:25599667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4337225/
Abstract

Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA (Piwi-interacting RNA), snoRNA (small nucleolar RNA), lincRNA (large non-coding intergenic RNA), SRP RNA (signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.

摘要

本单元所述的链特异性全转录组RNA测序可获取所有类型RNA的定量表达数据，包括但不限于微小RNA（miRNA）、Piwi相互作用RNA（piRNA）、小核仁RNA（snoRNA）、长链非编码基因间RNA（lincRNA）、信号识别颗粒RNA（SRP RNA）、转运RNA（tRNA）、线粒体RNA（mtRNA）和信使RNA（mRNA）。这些RNA类型的大小和性质与本文所述方法无关。采用这种方法可生成用于在Illumina平台上进行多重分析的条形码文库，但稍作修改后也可应用于其他平台。

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