Vázquez A J F, Cedillo M J, Quezada V J, Rivas A C, Morales E C L, Ayala E M E, Hernández M J, González R A, Aragón M A
Centro Universitario Temascaltepec, Universidad Autónoma del Estado de México, CP 50090 Toluca, Mexico.
Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico.
Anim Reprod Sci. 2015 Mar;154:29-38. doi: 10.1016/j.anireprosci.2014.12.009. Epub 2014 Dec 24.
Here we show the effects of repeated electroejaculation (EE) on mean values of motility, mitochondrial functionality, and expression of active caspases on goat sperm obtained by EE. Evaluations were done using CASA and flow cytometry. A strategy for identification of kinematic sperm subpopulations, when individual data of sperm are not provided by the CASA system, is provided. Fifty semen samples, five of each of ten adult creole goats, were obtained by electroejaculation. Mean values of total motility, progressive motility and flow cytometry evaluations were compared among EEs. Relationships among mean values of variables were investigated using Spearman correlation and principal component analysis (PCA). For identification of kinematic sperm subpopulations, PCA followed by hierarchical clustering was applied on data of the intervals provided automatically by the CASA system. Total motility does no change after repeated EE. Mean values of motility parameters and molecular markers were unrelated in multivariate space, but bivariate correlations were found. Values in upper and lower intervals defined clearly the sperm subpopulations, which had motility parameters changing over time. Taken together, our results show that repeated EE does not affect mean values of total motility, that molecular markers are not related with motility parameters, and that it is possible to identify kinematic sperm subpopulations when individual data, of motility parameters, are not provided by the CASA system.
在此，我们展示了重复电射精（EE）对通过EE获得的山羊精子活力、线粒体功能以及活性半胱天冬酶表达平均值的影响。使用计算机辅助精子分析（CASA）和流式细胞术进行评估。当CASA系统未提供精子个体数据时，提供了一种识别精子运动亚群的策略。通过电射精从十只成年克里奥尔山羊中获取了五十份精液样本，每只山羊五份。比较了不同电射精次数下的总活力、渐进性活力平均值以及流式细胞术评估结果。使用斯皮尔曼相关性和主成分分析（PCA）研究了变量平均值之间的关系。为了识别精子运动亚群，对CASA系统自动提供的时间间隔数据应用PCA后进行层次聚类。重复电射精后总活力没有变化。在多变量空间中，活力参数和分子标记的平均值不相关，但发现了双变量相关性。上下时间间隔内的值清晰地定义了精子亚群，其活力参数随时间变化。综上所述，我们的结果表明，重复电射精不影响总活力平均值，分子标记与活力参数无关，并且当CASA系统未提供活力参数的个体数据时，可以识别精子运动亚群。
