Mosca Fabio, Zaniboni Luisa, Sayed Ahmad Abdel, Iaffaldano Nicolaia, Soglia Dominga, Schiavone Achille, Cerolini Silvia
Dipartimento di Medicina Veterinaria, University of Milan, via dell'Università 6, 26900 Lodi, Italy.
Dipartimento di Agricoltura, Ambiente e Alimenti, University of Molise, via De Sanctis, 86100 Campobasso, Italy.
Animals (Basel). 2020 May 9;10(5):824. doi: 10.3390/ani10050824.
In seeking alternative cryoprotectants to glycerol for a reference chicken semen freezing procedure, the aim of the present study was to compare the effect of two concentrations of N-Methylacetamide (MA) and two thawing rates on the quality of frozen-thawed semen. Semen samples were diluted in Lake pre-freezing extender, including 0.1 M trehalose in presence of 6% or 9% MA, loaded into straws, frozen in nitrogen vapors, and stored in liquid nitrogen. The following thawing treatments were used: 5 °C for 100 s and 38 °C for 30 s. Sperm quality (cell membrane integrity, motility and kinetic parameters) was assessed before and after cryopreservation. The decrease of MA concentration from 9 to 6% improved sperm quality after freezing/thawing and this effect was dependent on thawing temperature. Decreasing the MA concentration from 9 to 6% improved the proportion of undamaged membrane, motile, and progressive motile sperm recovered after thawing at 5 °C for 100 s; in contrast, no effect of the MA concentration was observed thawing at 38 °C for 30 s. Therefore, the treatment with 6% MA and thawing at 5 °C for 100 s has given the best cryoprotective action. These results contribute to improve the efficacy of the current chicken semen cryopreservation procedures.
在为参考鸡精液冷冻程序寻找甘油替代冷冻保护剂时，本研究的目的是比较两种浓度的N - 甲基乙酰胺（MA）和两种解冻速率对冻融精液质量的影响。精液样本在Lake预冻稀释液中稀释，该稀释液在6%或9%的MA存在下含有0.1 M海藻糖，装入细管，在氮蒸气中冷冻，并储存在液氮中。采用以下解冻处理：5℃ 100秒和38℃ 30秒。在冷冻保存前后评估精子质量（细胞膜完整性、活力和动力学参数）。MA浓度从9%降至6%可提高冻融后精子质量，且这种效果取决于解冻温度。将MA浓度从9%降至6%可提高在5℃ 100秒解冻后回收的未受损膜、有活力和进行性运动精子的比例；相比之下，在38℃ 30秒解冻时未观察到MA浓度的影响。因此，用6%的MA处理并在5℃ 100秒解冻具有最佳的冷冻保护作用。这些结果有助于提高当前鸡精液冷冻保存程序的效果。
