Laboratorio de Pubertad, Facultad de Estudios Superiores Zaragoza, UNAM. AP 9-020, Unidad de Investigación en Biología de la Reproducción, Distrito Federal, CP 15000, México.
Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México Los Reyes Iztacala, Tlalnepantla, Estado de México, C.P. 54090, México.
Cytometry A. 2017 Nov;91(11):1096-1103. doi: 10.1002/cyto.a.23246. Epub 2017 Sep 20.
Here, we show the effects of sexual steroids, progesterone, testosterone, or estradiol on motility parameters of boar sperm. Sixteen commercial seminal doses, four each of four adult boars, were analyzed using computer assisted sperm analysis (CASA). Mean values of motility parameters were analyzed by bivariate and multivariate statistics. Principal component analysis (PCA), followed by hierarchical clustering, was applied on data of motility parameters, provided automatically as intervals by the CASA system. Effects of sexual steroids were described in the kinematic subpopulations identified from multivariate statistics. Mean values of motility parameters were not significantly changed after addition of sexual steroids. Multivariate graphics showed that sperm subpopulations were not sensitive to the addition of either testosterone or estradiol, but sperm subpopulations responsive to progesterone were found. Distribution of motility parameters were wide in controls but sharpened at distinct concentrations of progesterone. We conclude that kinematic sperm subpopulations responsive to progesterone are present in boar semen, and these subpopulations are masked in evaluations of mean values of motility parameters. © 2017 International Society for Advancement of Cytometry.
在这里，我们展示了性类固醇、孕酮、睾酮或雌二醇对猪精子运动参数的影响。使用计算机辅助精子分析（CASA）分析了 16 个商业精液剂量，每个剂量来自 4 头成年公猪。通过双变量和多变量统计分析了运动参数的平均值。主成分分析（PCA），随后是层次聚类，应用于运动参数的数据，由 CASA 系统自动提供间隔。从多变量统计中确定了性类固醇的运动子群的作用。添加性类固醇后，运动参数的平均值没有显著变化。多变量图形显示，精子亚群对添加睾酮或雌二醇不敏感，但发现对孕酮有反应的精子亚群。在对照中，运动参数的分布范围较宽，但在孕酮的特定浓度下变得更尖锐。我们得出结论，对孕酮有反应的运动精子亚群存在于公猪精液中，这些亚群在运动参数平均值的评估中被掩盖。©2017 国际细胞分析学会。
