Nour Moustapha, Stiger-Pouvreau Valérie, Guenneguez Alain, Meslet-Cladière Laurence, Cérantola Stéphane, Ali Ahmed, Simon Gaelle, Daher Abdourahman, Petek Sylvain
Univ Brest, Institut de Recherche pour le Développement (IRD), CNRS, Ifremer, LEMAR, IUEM, F-29280 Plouzane, France.
Centre d'Études et de Recherche de Djibouti, Institut des Sciences de la Vie ISV, Route de l'Aéroport, Haramous BP 486, Djibouti.
Mar Drugs. 2024 Dec 28;23(1):15. doi: 10.3390/md23010015.
This study focuses on developing innovative and eco-friendly purification methods for the isolation of bioactive compounds derived from , a brown abundant macroalga in Djibouti. Three distinct fractions, obtained via liquid-liquid extraction (LLE_FAE), solid-phase extraction (SPE_WE50), and flash chromatography (FC_EtOH20), were selected based on their high phenolic content and antioxidant activities. All fractions were also evaluated for their anti-ageing potential by assessing their ability to inhibit two vital skin-ageing enzymes, tyrosinase and elastase. Structural analysis by H-C HMBC NMR and LC-MS revealed a selectivity of phlorotannins depending on the purification methods. The LLE_FAE fraction exhibited greater structural complexity, including compounds such as phloroglucinol, diphlorethol/difucol, fucophlorethol and bifuhalol, which likely contribute to its enhanced bioactivity compared to the fractions obtained by FC_EtOH20 and SPE_WE50, which were also active and enriched only in phloroglucinol and fucophlorethol. These findings highlight the impact of purification techniques on the selective enrichment of specific bioactive compounds and demonstrated the interest of FC or SPE in producing active phlorotannin-enriched fractions. These two purification methods hold strong potential for innovative cosmeceutical applications. Results are discussed regarding the use of as a promising marine resource in Djibouti to be used for the development of cosmetic industry.
本研究聚焦于开发创新且环保的纯化方法，用于从吉布提一种丰富的棕色大型藻类中分离生物活性化合物。基于其高酚含量和抗氧化活性，选择了通过液液萃取（LLE_FAE）、固相萃取（SPE_WE50）和快速色谱法（FC_EtOH20）获得的三个不同馏分。通过评估它们抑制两种重要皮肤老化酶（酪氨酸酶和弹性蛋白酶）的能力，对所有馏分的抗老化潜力也进行了评估。通过H-C HMBC NMR和LC-MS进行的结构分析揭示了根据纯化方法不同，间苯三酚单宁具有选择性。LLE_FAE馏分表现出更大的结构复杂性，包括间苯三酚、二氯间苯二酚/二岩藻酚、岩藻间苯二酚和双褐藻酚等化合物，与通过FC_EtOH20和SPE_WE50获得的馏分相比，这些化合物可能有助于其增强的生物活性，后两者也具有活性且仅富含间苯三酚和岩藻间苯二酚。这些发现突出了纯化技术对特定生物活性化合物选择性富集的影响，并证明了FC或SPE在生产富含活性间苯三酚单宁馏分方面的价值。这两种纯化方法在创新的药妆应用方面具有强大潜力。讨论了将其作为吉布提一种有前景的海洋资源用于化妆品行业发展的相关结果。
