Department of Microbiology and Immunology, Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium; Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia; Basic Sciences Department, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia; Centro de Malária e Outras Doenc¸ as Tropicais and Unidade de Saúde Pública Internacional e Bioestatística, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal.
Department of Zoology, University of Oxford, South Parks Road, OX1-3PS, Oxford, United Kingdom.
J Clin Virol. 2015 Feb;63:38-41. doi: 10.1016/j.jcv.2014.12.010. Epub 2014 Dec 17.
Hepatitis B virus (HBV) has been classified into eight genotypes and forty subgenotypes. Genotype D of HBV is the most worldwide distributed genotype and HBV subgenotype D1 has been isolated from Iranian patients.
To characterize for the first time complete genomes of recently emerged non-D1 strains in Iran.
HBV complete genomes isolated from 9 Iranian HBV carriers were sequenced. Different diversities of the ORFs were mapped and evolutionary history relationships were investigated.
Phylogenetic analysis identified four D2 subgenotypes and five D3 subgenotypes of HBV in the studied patients. Of note, D2 strains clustered with strains from Lebanon and Syria. The time of the most recent common ancestor (TMRCA) of the first cluster of D2 was dated at 1953 (BCI=1926, 1976) while the second cluster was dated at 1947 (BCI=1911, 1978). All five Iranian D3 strains formed a monophyletic cluster with Indian strain and dated back to 1967 (BCI=1946, 1987). Surprisingly, two D3 strains had an adw2 subtype. Interestingly, more than 80% of the present strains showed precore mutations, while two isolates carried basal core promoter variation.
Iranian D2 and D3 isolates were introduced on at least two and one occasion in Iran and diverged from west and south Asian HBV strains, respectively. Considering the impact of the different (sub) genotypes on clinical outcome, exploring the distinct mutational patterns of Iranian D1 and non-D1 strains is of clinical importance.
乙型肝炎病毒（HBV）已分为 8 个基因型和 40 个亚型。HBV 基因型 D 是分布最广泛的基因型，HBV 亚型 D1 已从伊朗患者中分离出来。
首次对伊朗新出现的非 D1 株的完整基因组进行特征描述。
对 9 名伊朗 HBV 携带者的 HBV 完整基因组进行测序。对 ORF 的不同多样性进行了映射，并对进化史关系进行了研究。
系统进化分析在研究患者中鉴定出 4 种 D2 亚型和 5 种 D3 亚型的 HBV。值得注意的是，D2 株与来自黎巴嫩和叙利亚的株聚类。D2 第一簇的最近共同祖先（TMRCA）时间可追溯到 1953 年（BCI=1926，1976），而第二簇可追溯到 1947 年（BCI=1911，1978）。伊朗的 5 株 D3 株与印度株形成单系聚类，可追溯到 1967 年（BCI=1946，1987）。令人惊讶的是，两株 D3 株具有 adw2 亚型。有趣的是，目前超过 80%的株显示出前核心突变，而两株分离株携带基本核心启动子变异。
伊朗 D2 和 D3 分离株至少在伊朗传入两次和一次，并分别与西亚和南亚的 HBV 株分离。考虑到不同（亚）基因型对临床结果的影响，研究伊朗 D1 和非 D1 株的不同突变模式具有临床意义。
