Jeong Yun-Mi, Duanting Zhai, Hennig Holger, Samanta Animesh, Agrawalla Bikram Keshari, Bray Mark-Anthony, Carpenter Anne E, Chang Young-Tae
Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore.
Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.
Chem Biol. 2015 Feb 19;22(2):299-307. doi: 10.1016/j.chembiol.2014.11.018. Epub 2015 Jan 15.
Long-term real-time visualization of lysosomal dynamics has been challenging at the onset of mitosis due to the lack of fluorescent probes enabling convenient imaging of dividing cells. We developed a long-term real-time photostable mitotic or proliferating marker, CDy6, a BODIPY-derived compound of designation yellow 6, which labels lysosome. In long-term real-time, CDy6 displayed a sharp increase in intensity and change in localization in mitosis, improved photostability, and decreased toxicity compared with other widely used lysosomal and DNA markers, and the ability to label cells in mouse xenograft models. Therefore, CDy6 may open new possibilities to target and trace lysosomal contents during mitosis and to monitor cell proliferation, which can further our knowledge of the basic underlying biological mechanisms in the management of cancer.
由于缺乏能够方便地对分裂细胞进行成像的荧光探针，在有丝分裂开始时对溶酶体动力学进行长期实时可视化一直具有挑战性。我们开发了一种长期实时光稳定的有丝分裂或增殖标记物CDy6，一种命名为黄色6的硼二吡咯衍生化合物，它可标记溶酶体。与其他广泛使用的溶酶体和DNA标记物相比，CDy6在长期实时观察中显示出强度的急剧增加和有丝分裂过程中定位的变化，提高了光稳定性，降低了毒性，并且能够在小鼠异种移植模型中标记细胞。因此，CDy6可能为在有丝分裂期间靶向和追踪溶酶体内容物以及监测细胞增殖开辟新的可能性，这可以加深我们对癌症管理中基本生物学机制的了解。
