Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry , Sichuan University , Chengdu , 610064 , China.
Anal Chem. 2018 May 1;90(9):5873-5878. doi: 10.1021/acs.analchem.8b00590. Epub 2018 Apr 20.
The viscosity of lysosome is reported to be a key indicator of lysosomal functionality. However, the existing mechanical methods of viscosity measurement can hardly be applied at the cellular or subcellular level. Herein, a BODIPY-based two-photon fluorescent probe was presented for monitoring lysosomal viscosity with high spatial and temporal resolution. By installing two morpholine moieties to the fluorophore as target and rotational groups, the TICT effect between the two morpholine rings and the main fluorophore scaffold endowed the probe with excellent viscosity sensitivity. Moreover, Lyso-B succeeded in showing the impact of dexamethasone on lysosomal viscosity in real time.
溶酶体的黏度被报道为溶酶体功能的一个关键指标。然而，现有的力学方法几乎无法应用于细胞或亚细胞水平。在此，我们提出了一种基于 BODIPY 的双光子荧光探针，用于高时空分辨率监测溶酶体的黏度。通过在荧光团上安装两个吗啉基团作为靶标和旋转基团，两个吗啉环与主荧光团骨架之间的 TICT 效应赋予了探针优异的黏度敏感性。此外，Lyso-B 成功地实时显示了地塞米松对溶酶体黏度的影响。
