Gao Guangliang, Li Qin, Zhao Xianzhi, Ding Ning, Han Qing, Su Jian, Wang Qigui
Poultry Science Institute, Chongqing Academy of Animal Science, Chongqing, China.
Anim Sci J. 2015 Aug;86(8):800-5. doi: 10.1111/asj.12356. Epub 2015 Jan 20.
The Sichuan White goose is a Chinese breed well known for the quality of its meat. However, reproductive performance in this goose is not ideal, and little information is available regarding the abundance of transcripts. To better understand the molecular mechanism(s) underpinning prelaying and laying periods in the Sichuan white goose, high-throughput RNA sequencing was performed to analyze the transcriptome in the hypothalamus. After sequencing and annotation, 26,921 unigenes were obtained, with 48 transcripts up-regulated in the prelaying period and 180 transcripts up-regulated during the laying period. These transcripts were primarily related to diseases, cancers, signaling molecules and interactions, the nervous system and the immune system. Eight transcripts were selected for further analyses with quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The qRT-PCR results were mostly consistent with those from the high-throughput RNA sequencing. Among these transcripts, serine/threonine-protein kinase (AMPK), heat shock protein 70 (HSP70) and NADH dehydrogenase 1 (ND1) were differentially expressed during the prelaying and laying periods. The results of this study provide a useful resource for future studies examining the hypothalamus in geese.
四川白鹅是一种以肉质优良而闻名的中国鹅种。然而，这种鹅的繁殖性能并不理想，关于转录本丰度的信息也很少。为了更好地了解四川白鹅产蛋前期和产蛋期的分子机制，进行了高通量RNA测序以分析下丘脑的转录组。经过测序和注释，获得了26,921个单基因，其中48个转录本在产蛋前期上调，180个转录本在产蛋期上调。这些转录本主要与疾病、癌症、信号分子与相互作用、神经系统和免疫系统有关。选择了8个转录本进行定量逆转录聚合酶链反应（qRT-PCR）进一步分析。qRT-PCR结果与高通量RNA测序结果基本一致。在这些转录本中，丝氨酸/苏氨酸蛋白激酶（AMPK）、热休克蛋白70（HSP70）和NADH脱氢酶1（ND1）在产蛋前期和产蛋期差异表达。本研究结果为今后研究鹅下丘脑提供了有用的资源。
