Luan Xinhong, Cao Zhongzan, Xing Zhe, Liu Mei, Gao Ming, Meng Bo, Fan Ruiming
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, P.R. China.
PLoS One. 2017 Sep 25;12(9):e0185253. doi: 10.1371/journal.pone.0185253. eCollection 2017.
In this study, we performed a comprehensive evaluation of the proteomic profile of the pituitary gland of the Huoyan goose during the laying period compared to the pre-laying period using an iTRAQ-based approach. Protein samples were prepared from pituitary gland tissues of nine pre-laying period and nine laying period geese. Then the protein samples from three randomly selected geese within each period were pooled in equal amounts to generate one biological sample pool. We identified 684 differentially expressed proteins, including 418 up-regulated and 266 down-regulated proteins. GO annotation and KEGG pathway analyses of these proteins were conducted. Some of these proteins were found to be associated with hormone and neurotransmitter secretion and transport, neuropeptide signalling and GnRH signalling pathways, among others. Subsequently, the modification of the abundance of three proteins (prolactin, chromogranin-A and ITPR3) was verified using western blotting. Our results will provide a new source for mining genes and gene products related to the egg-laying performance of Huoyan geese, and may provide important information for the conservation and utilization of local goose breeds.
在本研究中，我们采用基于iTRAQ的方法，对产蛋期与产蛋前期火焰鹅垂体的蛋白质组图谱进行了全面评估。从9只产蛋前期和9只产蛋期鹅的垂体组织中制备蛋白质样品。然后将每个时期随机选取的3只鹅的蛋白质样品等量混合，以生成一个生物样品池。我们鉴定出684种差异表达蛋白，其中包括418种上调蛋白和266种下调蛋白。对这些蛋白进行了基因本体（GO）注释和京都基因与基因组百科全书（KEGG）通路分析。发现其中一些蛋白与激素和神经递质的分泌与运输、神经肽信号传导和促性腺激素释放激素（GnRH）信号通路等有关。随后，使用蛋白质免疫印迹法验证了三种蛋白（催乳素、嗜铬粒蛋白A和三磷酸肌醇受体3，ITPR3）丰度的变化。我们的结果将为挖掘与火焰鹅产蛋性能相关的基因和基因产物提供新的来源，并可能为地方鹅种的保护和利用提供重要信息。
