Lee Ji-Eun, Oh Jang-Hyun, Ku MyungHee, Kim Jueun, Lee Jung-Shin, Kang Sa-Ouk
Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Department of Molecular Biosciences, College of Biomedical Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do 200-701, Republic of Korea.
FEBS Lett. 2015 Feb 13;589(4):513-20. doi: 10.1016/j.febslet.2015.01.011. Epub 2015 Jan 17.
Ssn6 is a crucial regulator of morphological transition and virulence in the fungal pathogen Candida albicans. Ssn6 has previously been reported to act in complex with the transcriptional repressor Tup1. Here, we report that Ssn6 also interacts with the histone deacetylase Rpd31, independently of Tup1. The ssn6/rpd31 double mutant strain formed elongated filaments, but failed to form filament extension, and this coincided with the down-regulation of the filament extension gene UME6. Occupancy patterns of Ssn6 and Rpd31 differed at the promoters of UME6 and the metabolic gene INO1. These findings indicate that, in C. albicans, Ssn6 has dual roles in filament development, depending on the interaction with Rpd31.
Ssn6是真菌病原体白色念珠菌形态转变和毒力的关键调节因子。此前有报道称Ssn6与转录抑制因子Tup1形成复合物发挥作用。在此，我们报道Ssn6还能独立于Tup1与组蛋白脱乙酰酶Rpd31相互作用。ssn6/rpd31双突变菌株形成了细长的菌丝，但未能形成菌丝延伸，这与菌丝延伸基因UME6的下调相吻合。Ssn6和Rpd31在UME6启动子和代谢基因INO1上的占据模式不同。这些发现表明，在白色念珠菌中，Ssn6在菌丝发育中具有双重作用，这取决于与Rpd31的相互作用。
