Taiwe G S, Moto F C O, Ayissi E R M, Ngoupaye G T, Njapdounke J S K, Nkantchoua G C N, Kouemou N, Omam J P O, Kandeda A K, Pale S, Pahaye D, Ngo Bum E
Department of Zoology and Animal Physiology, Faculty of Sciences, University of Buea, P.O. Box 63, Buea, Cameroon.
Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon.
Epilepsy Behav. 2015 Feb;43:100-8. doi: 10.1016/j.yebeh.2014.11.022. Epub 2015 Jan 16.
Feretia apodanthera Del. (Rubiaceae) is extensively used in ethnomedicine in Cameroon and Nigeria for epilepsy, febrile convulsions, and rheumatic pains and for enhancing cognitive performance. The aim of the present study was to examine the effects of a lyophilized aqueous extract of F. apodanthera on the course of kindling development, kindling-induced learning deficit, oxidative stress markers, and cholinesterase activity in pentylenetetrazole (PTZ)-kindled mice. Pentylenetetrazole, 30mg/kg, induced kindling in mice after 30.00±1.67days. The aqueous extract of F. apodanthera showed dose-dependent antiseizure effects. Feretia apodanthera (150-200mg/kg) significantly increased the latency to myoclonic jerks, clonic seizures, and generalized tonic-clonic seizures. The extract also improved the seizure score and decreased the number of myoclonic jerks. Pentylenetetrazole kindling induced significant oxidative stress and cognitive impairment which were reversed by pretreatment with F. apodanthera in a dose-dependent manner. The significant decrease in cholinesterase activity observed in the PTZ-kindled mice was reversed by pretreatment with the F. apodanthera extract. The results indicated that pretreatment with the aqueous extract of F. apodanthera antagonizes seizures, oxidative stress, and cognitive impairment in PTZ-kindled mice. The aqueous extract of F. apodanthera also showed anxiolytic activities, but the inhibition of memory impairment was not attributed to the anxiolytic activities of the plant. These results thus suggest the potential of F. apodanthera as an adjuvant in epilepsy both to prevent seizures as well as to protect against seizure-induced oxidative stress and memory impairment.
费氏龙船花（茜草科）在喀麦隆和尼日利亚的民族医学中被广泛用于治疗癫痫、高热惊厥、风湿疼痛以及提高认知能力。本研究的目的是考察费氏龙船花冻干水提取物对戊四氮（PTZ）点燃小鼠的点燃发展过程、点燃诱导的学习缺陷、氧化应激标志物及胆碱酯酶活性的影响。30mg/kg的戊四氮在30.00±1.67天后诱导小鼠发生点燃。费氏龙船花的水提取物呈现出剂量依赖性的抗癫痫作用。费氏龙船花（150 - 200mg/kg）显著延长了肌阵挛性抽搐、阵挛性发作和全身性强直 - 阵挛性发作的潜伏期。该提取物还改善了癫痫发作评分并减少了肌阵挛性抽搐的次数。戊四氮点燃诱导了显著的氧化应激和认知障碍，而费氏龙船花预处理以剂量依赖性方式逆转了这些情况。在PTZ点燃小鼠中观察到的胆碱酯酶活性显著降低，被费氏龙船花提取物预处理所逆转。结果表明，费氏龙船花水提取物预处理可拮抗PTZ点燃小鼠的癫痫发作、氧化应激和认知障碍。费氏龙船花的水提取物还表现出抗焦虑活性，但记忆损伤的抑制并非归因于该植物的抗焦虑活性。因此，这些结果表明费氏龙船花作为癫痫辅助治疗药物具有预防癫痫发作以及对抗发作诱导的氧化应激和记忆损伤的潜力。
