Ngaibi Joseph, Kandeda Antoine Kavaye, Nguezeye Yvette, Wangbara Tchang Alkali, Gaoudji Lamido, Taiwe Germain Sotoing, Bum Elisabeth Ngo
Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon, P.O. Box 67, Dschang, Cameroon.
Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Heliyon. 2024 Oct 17;10(20):e39483. doi: 10.1016/j.heliyon.2024.e39483. eCollection 2024 Oct 30.
Moldenke (Verbenaceae) is an aromatic plant used as a popular medicine with antidepressant, antispasmodic, antifungal, anti-inflammatory, and antioxidant properties. In this study, we explored the effects of in mice chronic model of temporal lobe epilepsy induced by pilocarpine and kindled with pentylenetetrazol. Mice were divided into 7 groups of 10 animals, and received a single dose of pilocarpine (360 mg/kg, i.p.), 20 min after the administration of N-methyl-scopolamine (1 mg/kg, i.p). Thirty days after the induction of , animals were daily treated for 60 days with distilled water (10 mL/kg, ) for the negative control group, extract (23.07, 57.69, 115.39 and 230.78 mg/kg, ) for the test groups, and sodium valproate (300 mg/kg, i.p) for the positive control group. On every 10th day, animals were injected with a sub-convulsive dose of pentylenetetrazol (15 mg/kg, i.p) 1 h after the administration of the various treatments to assess the susceptibility of animals to seizures. At the end of behavioural tests, animals were sacrificed and the level of inflammatory cytokines was assessed in the hippocampus. The plant extract reduced (p < 0.001) the occurrence of seizures and the number of spontaneous recurrent seizures induced by pilocarpine in mice. It ameliorated the levels of inflammatory cytokines (TNF-α, INF- γ, IL-1β, IL-6, and IL-10) in the hippocampus. The studies show that have a high amount of total phenolic content, flavonoids and tannins and also have some good antioxidant properties. These results suggest that aqueous extracts has the potential to be a promising complementary and alternative medicine for the treatment of epilepsy, due to its antiepileptic, anti-inflammatory and antioxidant effects.
莫尔登克（马鞭草科）是一种芳香植物，作为一种常用药物，具有抗抑郁、解痉、抗真菌、抗炎和抗氧化特性。在本研究中，我们探讨了其对匹鲁卡品诱导并用戊四氮点燃的小鼠慢性颞叶癫痫模型的影响。将小鼠分为7组，每组10只动物，在腹腔注射N - 甲基东莨菪碱（1 mg/kg）20分钟后，腹腔注射单剂量匹鲁卡品（360 mg/kg）。癫痫诱导30天后，阴性对照组动物每天用蒸馏水（10 mL/kg）处理60天，试验组用提取物（23.07、57.69、115.39和230.78 mg/kg）处理60天，阳性对照组用丙戊酸钠（300 mg/kg，腹腔注射）处理60天。每隔10天，在给予各种处理1小时后，给动物注射亚惊厥剂量的戊四氮（15 mg/kg，腹腔注射），以评估动物对癫痫发作的易感性。行为测试结束时，处死动物并评估海马中炎性细胞因子的水平。该植物提取物降低了（p < 0.001）小鼠中匹鲁卡品诱导的癫痫发作次数和自发性复发性癫痫发作的数量。它改善了海马中炎性细胞因子（TNF-α、INF-γ、IL-1β、IL-6和IL-10）的水平。研究表明，该提取物含有大量的总酚含量、黄酮类化合物和单宁，并且还具有一些良好的抗氧化特性。这些结果表明，由于其抗癫痫、抗炎和抗氧化作用，该植物水提取物有潜力成为一种有前景的治疗癫痫的补充和替代药物。
