Brooks A N, Challis J R
Lawson Research Institute, St. Joseph's Health Centre, London, Ontario, Canada.
Peptides. 1989 Nov-Dec;10(6):1291-3. doi: 10.1016/0196-9781(89)90024-7.
Intravenous administration of equimolar doses of CRF (30 micrograms) and AVP (6 micrograms) to mature female sheep resulted in elevated plasma concentrations of ACTH and cortisol. Simultaneous administration of equimolar amounts of CRF and AVP resulted in a greater ACTH response compared with the sum of the responses to CRF or AVP given independently. Intravenous bolus administration of the endogenous opioid, Met-enkephalin (2.5 mg), and its potent and long-acting analogue, [D-Ala2,N-Phe4,Met(O)ol5]-enkephalin [FK33-824 (250 micrograms)], did not alter ACTH or cortisol secretion. Furthermore, naloxone, an opioid receptor antagonist given alone or concurrently with Met-enkephalin or FK33-824, was without effect. Pituitary-adrenal responses to CRF were unaltered by simultaneous administration of Met-enkephalin, FK33-824 or naloxone. These results suggest that in the sheep, opioid involvement in the tonic regulation of pituitary-adrenal function is absent. However, CRF and AVP may act alone or in synergy to control the release of biologically active ACTH from the sheep pituitary gland.
对成年雌性绵羊静脉注射等摩尔剂量的促肾上腺皮质激素释放因子（CRF，30微克）和血管升压素（AVP，6微克），会导致血浆促肾上腺皮质激素（ACTH）和皮质醇浓度升高。与单独给予CRF或AVP的反应之和相比，同时给予等摩尔量的CRF和AVP会导致更大的ACTH反应。静脉推注内源性阿片样物质甲硫氨酸脑啡肽（2.5毫克）及其强效长效类似物[D-丙氨酸2，N-苯丙氨酸4，甲硫氨酸（O）醇5]-脑啡肽[FK33-824，250微克]，不会改变ACTH或皮质醇的分泌。此外，单独给予或与甲硫氨酸脑啡肽或FK33-824同时给予的阿片受体拮抗剂纳洛酮没有效果。同时给予甲硫氨酸脑啡肽、FK33-824或纳洛酮不会改变垂体-肾上腺对CRF的反应。这些结果表明，在绵羊中，阿片样物质不参与垂体-肾上腺功能的紧张性调节。然而，CRF和AVP可能单独或协同作用，以控制绵羊垂体中生物活性ACTH的释放。
