Shakeela Qismat, Shehzad Ahmed, Tang Kaihao, Zhang Yunhui, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1186-1192. doi: 10.1099/ijs.0.000078. Epub 2015 Jan 20.
A novel marine bacterium isolated from the intestine of cultured flounder (Paralichthys olivaceus) was studied by using a polyphasic taxonomic approach. The isolate was Gram-stain-negative, pleomorphic, aerobic, yellow and oxidase- and catalase-negative. Phylogenetic analysis of 16S rRNA gene sequences indicated that isolate Th6(T) formed a distinct branch within the family Flavobacteriaceae and showed 96.6% similarity to its closest relative, Bizionia hallyeonensis T-y7(T). The DNA G+C content was 29 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C(15 : 1) G, iso-C(15 : 0), iso-C(15 : 0) 3-OH, iso-C(17 : 0) 3-OH and summed feature 3 (C(15 : 1)ω6c and/or C(16 : 1)ω7c). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, the novel bacterium has been assigned to a novel species of a new genus for which the name Ichthyenterobacterium magnum gen. nov., sp. nov. is proposed. The type strain is Th6(T) ( = JCM 18636(T) = KCTC 32140(T)).
采用多相分类学方法对从养殖牙鲆（Paralichthys olivaceus）肠道中分离出的一种新型海洋细菌进行了研究。该分离株革兰氏染色阴性，形态多样，好氧，黄色，氧化酶和过氧化氢酶阴性。16S rRNA基因序列的系统发育分析表明，分离株Th6(T)在黄杆菌科内形成一个独特的分支，与其最接近的亲缘种Bizionia hallyeonensis T-y7(T)的相似性为96.6%。DNA G+C含量为29 mol%。主要呼吸醌为MK-6。主要脂肪酸为异-C(15:1) G、异-C(15:0)、异-C(15:0) 3-OH、异-C(17:0) 3-OH和总和特征3（C(15:1)ω6c和/或C(16:1)ω7c）。基于表型、化学分类和系统发育特征，该新型细菌被归入一个新属的新物种，为此提出新属名Ichthyenterobacterium，新种名magnum。模式菌株为Th6(T)（=JCM 18636(T)=KCTC 32140(T)）。
