Addou Nariman Ammara, Schumann Peter, Spröer Cathrin, Ben Hania Wajdi, Hacene Hocine, Fauque Guy, Cayol Jean-Luc, Fardeau Marie-Laure
Laboratoire de Biologie Cellulaire et Moléculaire (Equipe de Microbiologie), Université des Sciences et de la Technologie Houari Boumediène, BP 32, El Alia 16111 Bab Ezzouar, Algiers, Algeria.
Aix Marseille Université, IRD, Université de Toulon, CNRS, MIO UM 110, 13288 Marseille, France.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1172-1179. doi: 10.1099/ijs.0.000075. Epub 2015 Jan 20.
A novel filamentous, endospore-forming, thermophilic and moderately halophilic bacterium designated strain Nari2A(T) was isolated from soil collected from an Algerian salt lake, Chott Melghir. The novel isolate was Gram-staining-positive, aerobic, catalase-negative and oxidase-positive. Optimum growth occurred at 50-55 °C, 7-10% (w/v) NaCl and pH 7-8. The strain exhibited 95.4, 95.4 and 95.2% 16S rRNA gene sequence similarity to Thalassobacillus devorans G19.1(T), Sediminibacillus halophilus EN8d(T) and Virgibacillus kekensis YIM-kkny16(T), respectively. The major menaquinone was MK-7. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were iso-C(15 : 0) and iso-C(17 : 0). The DNA G+C content was 41.9 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain Nari2A(T) is considered to represent a novel species of a new genus in the family Bacillaceae , order Bacillales , for which the name Melghiribacillus thermohalophilus gen. nov., sp. nov. is proposed. The type strain of Melghiribacillus thermohalophilus is Nari2A(T) ( = DSM 25894(T) = CCUG 62543(T)).
