Liu Y, Müllbacher A
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Immunol Cell Biol. 1989 Dec;67 ( Pt 6):413-20. doi: 10.1038/icb.1989.58.
The cellular requirements for the generation and activation of anti-influenza memory class I MHC restricted Tc cell responses were studied by selectively reconstituting lethally irradiated mice. The generation of memory Tc cells was investigated by using unprimed splenocytes to reconstitute infected, lethally irradiated mice; the activation of memory Tc cells was tested by using primed splenocytes to reconstitute uninfected, lethally irradiated mice. It is shown here that depletion of CD4+ T cells from donor cells did not reduce Tc cell responses in recipient mice. Depletion of CD8+ T cells from donor splenocytes prevented the memory Tc cell responses. Thus depletion of CD4+ T cells had no effect on the generation and activation of the memory Tc cells in vivo.
通过选择性地重建经致死剂量照射的小鼠，研究了产生和激活抗流感记忆性I类主要组织相容性复合体（MHC）限制性细胞毒性T细胞（Tc细胞）反应的细胞需求。通过使用未致敏的脾细胞重建受感染、经致死剂量照射的小鼠来研究记忆Tc细胞的产生；通过使用致敏的脾细胞重建未受感染、经致死剂量照射的小鼠来测试记忆Tc细胞的激活。结果表明，供体细胞中CD4 + T细胞的耗竭不会降低受体小鼠中的Tc细胞反应。供体脾细胞中CD8 + T细胞的耗竭阻止了记忆Tc细胞反应。因此，CD4 + T细胞的耗竭对体内记忆Tc细胞的产生和激活没有影响。
