The generation and activation of memory class I MHC restricted cytotoxic T cell responses to influenza A virus in vivo do not require CD4+ T cells.

The cellular requirements for the generation and activation of anti-influenza memory class I MHC restricted Tc cell responses were studied by selectively reconstituting lethally irradiated mice. The generation of memory Tc cells was investigated by using unprimed splenocytes to reconstitute infected, lethally irradiated mice; the activation of memory Tc cells was tested by using primed splenocytes to reconstitute uninfected, lethally irradiated mice. It is shown here that depletion of CD4+ T cells from donor cells did not reduce Tc cell responses in recipient mice. Depletion of CD8+ T cells from donor splenocytes prevented the memory Tc cell responses. Thus depletion of CD4+ T cells had no effect on the generation and activation of the memory Tc cells in vivo.