Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Center for Water Quality Research, Institute of Environmental Research, Tehran University of Medical Sciences, Tehran, Iran.
J Environ Health Sci Eng. 2014 Dec 24;12(1):150. doi: 10.1186/s40201-014-0150-2. eCollection 2014.
Use of earthworm to eliminate the phenanthrene from the soil (bioaccumulation) is developed as an economical method. Bioaugmentation of microorganism was used for promotion of bioaccumulation by earthworm. The aim of this study was to determine the bioaccumulation or biodegradation of phenanthrene by Eisenia fetida and bacterial consortium in polluted soil.
The amount of 0.4 kg of the polluted soil in the ratio of 10 and 30 mg phenanthrene per kg of dry soil was transferred into each pot. Afterwards, bacteria and earthworms were added to each pot in separate and combination. The samples were kept under field conditions, and the retention concentrations of phenanthrene were analyzed after 8 weeks.
Results showed that the Eisenia fetida was able to significantly remove phenanthrene from the polluted soil samples. Bioaccumulation and bioaugmentation alone have the removal efficiency of 60.24% and 50.3%, respectively. In the combined mode, phenanthrene removal efficiency was 63.81%.
The current study indicated that the use of earthworms, could improve both phenanthrene bioavailability and microbial activity, which led to enhancing removal of carbon-based pollutants.
利用蚯蚓从土壤中去除菲（生物积累）被开发为一种经济的方法。微生物的生物增强被用于促进蚯蚓的生物积累。本研究的目的是确定污染土壤中赤子爱胜蚓和细菌混合物对菲的生物积累或生物降解作用。
将 0.4 公斤受污染的土壤以每公斤干土 10 和 30 毫克菲的比例转移到每个锅中。然后，将细菌和蚯蚓分别添加到每个锅中。将样品置于田间条件下，8 周后分析菲的保留浓度。
结果表明，赤子爱胜蚓能够显著去除污染土壤样品中的菲。生物积累和生物增强单独的去除效率分别为 60.24%和 50.3%。在组合模式下，菲的去除效率为 63.81%。
本研究表明，使用蚯蚓可以提高菲的生物利用度和微生物活性，从而增强对碳基污染物的去除。
