Xu Xinyun, Li Xueyu, Liu Yuefeng
Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014 Dec;32(12):881-6.
To investigate whether cellular immunity and humoral immunity are involved in trichlorethylene (TCE)-induced mixed allergy, then provide the scientific basis for the mechanism of this disease.
Guinea pigs and rats were tested for this study by application of guinea pig maximization test (GPMT), the animals were randomly divided into negative control, positive control and TCE treatment groups. Animals of these groups were administrated with olive oil, 2, 4-dinitrochlorobenzene (DNCB), and TCE, respectively, by intradermal injection. After TCE administration, rat peripheral blood samples were collected by flow cytometry to detect lymphocytes CD3⁺, CD4⁺, CD8⁺. Guinea pig peripheral blood samples were collected to detect the levels of IgG, IgA, IgM, C3, C4, and the spleens were taken out from guinea pigs after various treatment, mRNA expression of GATA3, T-bet, CTLA4 and Foxp3 in lymphocytes of guinea pig spleen was detected by real-time fluorescent PCR assay. Additionally, TCE allergic dermatitis patients were selected for the study, the peripheral blood samples were collected from the TCE patients group and control group, quantitative PCR was applied to detect mRNA expression of immune-related genes Foxp3, GATA3, CTLA4, T-bet.
TCE induced obvious skin allergic reaction in guinea pigs, the sensitization rate was 83.3%, IgG levels in TCE group and positive control increased significantly. Additionally, mRNA expression levels of GATA3, T-bet, CTLA4 significantly elevated in TCE group and positive control, but Foxp3 mRNA levels decreased. The lymphocytes CD3⁺ ratio in TCE group and positive control of rats was higher than that in negative control, we found that there was no statistical difference of CD4⁺, CD8⁺, CD4⁺/CD8⁺ between TCE group and negative control of rats. The mRNA expression levels of Foxp3, GATA3, CTLA4 in TCE patients increased by 115%, 97%, 241%, respectively as compared with the control, T-bet levels decreased by 47%when compared with the control.
TCE could induce obvious changes of cellular immunity and humoral immunity in guinea pigs, rats, and TCE patients, these findings indicated that TCE-induced immunological disorder belongs to the mixed allergy with involvment of cellular immunity and humoral immunity, the mixed allergy might be type IV and type II allergy.
探讨细胞免疫和体液免疫是否参与三氯乙烯（TCE）诱导的混合性过敏反应，为该疾病的发病机制提供科学依据。
采用豚鼠最大化试验（GPMT）对豚鼠和大鼠进行检测，将动物随机分为阴性对照组、阳性对照组和TCE处理组。分别对这些组的动物进行皮内注射橄榄油、2,4-二硝基氯苯（DNCB）和TCE。给予TCE后，通过流式细胞术采集大鼠外周血样本检测淋巴细胞CD3⁺、CD4⁺、CD8⁺。采集豚鼠外周血样本检测IgG、IgA、IgM、C3、C4水平，并在不同处理后取出豚鼠脾脏，采用实时荧光定量PCR法检测豚鼠脾脏淋巴细胞中GATA3、T-bet、CTLA4和Foxp3的mRNA表达。此外，选取TCE过敏性皮炎患者进行研究，采集TCE患者组和对照组的外周血样本，应用定量PCR检测免疫相关基因Foxp3、GATA3、CTLA4、T-bet的mRNA表达。
TCE可诱导豚鼠出现明显的皮肤过敏反应，致敏率为83.3%，TCE组和阳性对照组的IgG水平显著升高。此外，TCE组和阳性对照组中GATA3、T-bet、CTLA4的mRNA表达水平显著升高，但Foxp3 mRNA水平降低。TCE组和大鼠阳性对照组的淋巴细胞CD3⁺比例高于阴性对照组，发现TCE组与大鼠阴性对照组之间CD4⁺、CD8⁺、CD4⁺/CD8⁺无统计学差异。与对照组相比，TCE患者中Foxp3、GATA3、CTLA4的mRNA表达水平分别升高了115%、97%、241%，T-bet水平与对照组相比降低了47%。
TCE可诱导豚鼠、大鼠及TCE患者细胞免疫和体液免疫发生明显变化，这些结果表明TCE诱导的免疫紊乱属于细胞免疫和体液免疫共同参与的混合性过敏反应，该混合性过敏反应可能为Ⅳ型和Ⅱ型过敏。
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