Jiang Shu-Ping, Lin Bing-Qi, Zhou Xing-Qiang, Li Min-Hua, Feng Zhen-Cheng, Qin Yue-Ying, Lin Shi-Qi, Zhou Zi-Qing, Peng Yang, Li Lian
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China.
Department of Otorhinolaryngology Head and Neck Surgery, Huizhou Central People's Hospital, Huizhou, People's Republic of China.
Ther Clin Risk Manag. 2025 Jun 24;21:975-986. doi: 10.2147/TCRM.S526727. eCollection 2025.
Respiratory inflammatory and infectious diseases continue to impose a substantial global health burden, compounded by persistent gaps in understanding their pathogenic mechanisms and limited therapeutic advancements. To address these challenges, this review systematically analyzed literature from PubMed, Web of Science, and Scopus databases (2005-2025) to evaluate the evolution and applications of airway organoid models in respiratory disease research. Key findings include: (1) the convergence of traditional culture techniques with advanced methodologies - including 3D matrix embedding, bioprinting and organoids-on-chips technologies - has enabled unprecedented recapitulation of human airway architecture and multicellular interactions; (2) these novel models provide unique insights into disease pathogenesis, host-microbe dynamics, and drug response variability; (3) the inherent capacity to maintain native cellular diversity and disease-associated phenotypes positions airway organoids as crucial platforms for personalized medicine approaches. Collectively, these advances establish airway organoids as transformative tools that bridge conventional in vitro models and clinical reality. Looking ahead, coupling organs-on-chips platforms with microgravity culture and single-cell lineage tracing will catalyze fundamental breakthroughs in respiratory disease research.
呼吸道炎症性和感染性疾病继续给全球健康带来沉重负担，对其致病机制的理解存在持续差距以及治疗进展有限使这一负担更加复杂。为应对这些挑战，本综述系统分析了来自PubMed、科学网和Scopus数据库（2005 - 2025年）的文献，以评估气道类器官模型在呼吸道疾病研究中的演变和应用。主要发现包括：（1）传统培养技术与先进方法（包括3D基质包埋、生物打印和芯片上类器官技术）的融合，实现了对人类气道结构和多细胞相互作用前所未有的重现；（2）这些新型模型为疾病发病机制、宿主 - 微生物动态以及药物反应变异性提供了独特见解；（3）维持天然细胞多样性和疾病相关表型的内在能力使气道类器官成为个性化医疗方法的关键平台。总体而言，这些进展确立了气道类器官作为连接传统体外模型与临床实际的变革性工具。展望未来，将芯片上器官平台与微重力培养和单细胞谱系追踪相结合，将推动呼吸道疾病研究取得根本性突破。
