Pati Falguni, Jang Jinah, Ha Dong-Heon, Won Kim Sung, Rhie Jong-Won, Shim Jin-Hyung, Kim Deok-Ho, Cho Dong-Woo
1] Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam ro, Nam-gu, Pohang, Kyungbuk 790-784, South Korea [2].
1] Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam ro, Nam-gu, Pohang, Kyungbuk 790-784, South Korea [2] Department of Bioengineering, University of Washington, Seattle, Waltham 98195, USA [3].
Nat Commun. 2014 Jun 2;5:3935. doi: 10.1038/ncomms4935.
The ability to print and pattern all the components that make up a tissue (cells and matrix materials) in three dimensions to generate structures similar to tissues is an exciting prospect of bioprinting. However, the majority of the matrix materials used so far for bioprinting cannot represent the complexity of natural extracellular matrix (ECM) and thus are unable to reconstitute the intrinsic cellular morphologies and functions. Here, we develop a method for the bioprinting of cell-laden constructs with novel decellularized extracellular matrix (dECM) bioink capable of providing an optimized microenvironment conducive to the growth of three-dimensional structured tissue. We show the versatility and flexibility of the developed bioprinting process using tissue-specific dECM bioinks, including adipose, cartilage and heart tissues, capable of providing crucial cues for cells engraftment, survival and long-term function. We achieve high cell viability and functionality of the printed dECM structures using our bioprinting method.
能够在三维空间中打印和构建构成组织的所有组件（细胞和基质材料）以生成类似于组织的结构，是生物打印令人兴奋的前景。然而，迄今为止用于生物打印的大多数基质材料无法体现天然细胞外基质（ECM）的复杂性，因此无法重构细胞的固有形态和功能。在此，我们开发了一种用新型脱细胞细胞外基质（dECM）生物墨水进行生物打印载细胞构建体的方法，这种生物墨水能够提供有利于三维结构化组织生长的优化微环境。我们展示了使用组织特异性dECM生物墨水（包括脂肪、软骨和心脏组织）所开发的生物打印过程的多功能性和灵活性，这些生物墨水能够为细胞植入、存活和长期功能提供关键线索。使用我们的生物打印方法，我们实现了打印的dECM结构的高细胞活力和功能。
