Bio-Manufacturing Programme, Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), 71 Nanyang Drive, 638075, Singapore. Singapore Centre for 3D Printing (SC3DP), School of Mechanical and Aerospace Engineering, Nanyang Technological University (NTU), 50 Nanyang Avenue, 639798, Singapore.
Biofabrication. 2018 Jan 23;10(2):025005. doi: 10.1088/1758-5090/aa9e1e.
Three-dimensional (3D) pigmented human skin constructs have been fabricated using a 3D bioprinting approach. The 3D pigmented human skin constructs are obtained from using three different types of skin cells (keratinocytes, melanocytes and fibroblasts from three different skin donors) and they exhibit similar constitutive pigmentation (pale pigmentation) as the skin donors. A two-step drop-on-demand bioprinting strategy facilitates the deposition of cell droplets to emulate the epidermal melanin units (pre-defined patterning of keratinocytes and melanocytes at the desired positions) and manipulation of the microenvironment to fabricate 3D biomimetic hierarchical porous structures found in native skin tissue. The 3D bioprinted pigmented skin constructs are compared to the pigmented skin constructs fabricated by conventional a manual-casting approach; in-depth characterization of both the 3D pigmented skin constructs has indicated that the 3D bioprinted skin constructs have a higher degree of resemblance to native skin tissue in term of the presence of well-developed stratified epidermal layers and the presence of a continuous layer of basement membrane proteins as compared to the manually-cast samples. The 3D bioprinting approach facilitates the development of 3D in vitro pigmented human skin constructs for potential toxicology testing and fundamental cell biology research.
使用三维（3D）生物打印方法制造了三维有色人种皮肤构建体。 3D 有色人种皮肤构建体是使用三种不同类型的皮肤细胞（来自三个不同皮肤供体的角质形成细胞，黑素细胞和成纤维细胞）获得的，它们表现出与皮肤供体相似的固有色素沉着（浅色素沉着）。两步按需滴注生物打印策略有利于细胞液滴的沉积，以模拟表皮黑色素单元（期望位置处角质形成细胞和黑素细胞的预定义图案化），并操纵微环境以制造天然皮肤组织中发现的 3D 仿生分层多孔结构。将 3D 生物打印的有色人种皮肤构建体与通过传统手动铸造方法制造的有色人种皮肤构建体进行比较；对这两种 3D 有色人种皮肤构建体进行了深入的表征，结果表明，与手动铸造的样本相比，3D 生物打印的皮肤构建体在表皮分层良好的分层和连续的基膜蛋白层的存在方面，与天然皮肤组织具有更高的相似性。 3D 生物打印方法有利于开发用于潜在毒理学测试和基础细胞生物学研究的 3D 体外有色人种皮肤构建体。
