Li Ai-Hua, Zhou Yu-Guang
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1199-1206. doi: 10.1099/ijs.0.000080. Epub 2015 Jan 21.
Three Gram-staining-negative, strictly aerobic, non-pigmented, non-motile, rod-shaped bacterial strains, SP32(T) ( = SLM-1(T)), SR68 ( = SLM-3) and SP95 ( = SLM-2), were isolated from two water samples of a cold-water lake in Xinjiang province, China. Growth was observed at 4-25 °C and pH 6.0-9.0, and optimum growth occurred at 18-20 °C and at pH 7.0-7.5. Phylogenetic analysis of 16S rRNA gene sequences revealed that these isolates belonged to the family Rhodobacteraceae , but formed an evolutionary lineage distinct from other species of this family with validly published names. Strain SP32(T) showed the highest 16S rRNA gene sequence similarity (96.7%) to Rhodobacter veldkampii ATCC 35703(T), and the similarity to members of the genera Defluviimonas , Haematobacter and Pseudorhodobacter was respectively 95.8-96.4, 96.0-96.1 and 95.3-96.1%. The genomic DNA G+C content of strain SP32(T) was 67.6 mol%. The major fatty acids (>5%) were summed feature 8 (C(18 : 1)ω7c/C(18 : 1)ω6c) and11-methyl C(18 : 1)ω7c. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified glycolipid and one unidentified polar lipid were the main polar lipids. Ubiquinone 10 (Q-10) was the sole respiratory quinone. Strain SP32(T) did not produce photosynthetic pigments and did not contain the gene pufM, by which it differed from the phototrophic species of the family Rhodobacteraceae . Based on its distinct phenotypic, chemotaxonomic and phylogenetic properties, strain SP32(T) represents a novel species in a novel genus within the family Rhodobacteraceae , for which we propose the name Frigidibacter albus gen. nov., sp. nov. The type strain of Frigidibacter albus is strain SP32(T) ( = SLM-1(T) = CGMCC 1.13995(T) = NBRC 109671(T)).
从中国新疆一个冷水湖的两份水样中分离出三株革兰氏染色阴性、严格需氧、无色素、不运动的杆状细菌菌株，即SP32(T)（=SLM-1(T)）、SR68（=SLM-3）和SP95（=SLM-2）。在4-25℃和pH 6.0-9.0条件下可观察到生长，最适生长温度为18-20℃，最适pH值为7.0-7.5。对16S rRNA基因序列的系统发育分析表明，这些分离株属于红杆菌科，但形成了一个与该科其他有效发表名称的物种不同的进化谱系。菌株SP32(T)与维氏红杆菌ATCC 35703(T)的16S rRNA基因序列相似性最高（96.7%），与脱氮单胞菌属、血杆菌属和假红杆菌属成员的相似性分别为95.8-96.4%、96.0-96.1%和95.3-96.1%。菌株SP32(T)的基因组DNA G+C含量为67.6 mol%。主要脂肪酸（>5%）为总和特征8（C(18 : 1)ω7c/C(18 : 1)ω6c）和11-甲基C(18 : 1)ω7c。磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱、一种未鉴定的糖脂和一种未鉴定的极性脂是主要的极性脂。泛醌10（Q-10）是唯一的呼吸醌。菌株SP32(T)不产生光合色素，也不含有pufM基因，因此与红杆菌科的光合营养物种不同。基于其独特的表型、化学分类和系统发育特性，菌株SP32(T)代表红杆菌科一个新属中的一个新物种，我们将其命名为白色嗜冷杆菌属新属，新种。白色嗜冷杆菌的模式菌株是SP32(T)（=SLM-1(T)=CGMCC 1.13995(T)=NBRC 109671(T)）。
