State Key Laboratory of Microbial Technology/Institute of Marine Science and Technology, Shandong University, Qingdao 266237, PR China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6155-6162. doi: 10.1099/ijsem.0.004512.
A Gram-stain-negative, aerobic, ovoid-rod-shaped bacterium, designated strain SM1903, was isolated from surface seawater of the Mariana Trench. The strain grew at 15-37 °C (optimum, 35 °C) and with 1-15 % (optimum, 4 %) NaCl. It hydrolysed aesculin but did not reduce nitrate to nitrite and hydrolyse Tween 80. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1903 formed a separate lineage within the family , sharing the highest 16S rRNA gene sequence similarity with type strains of (95.7 %) and (95.7 %). In phylogenetic trees based on single-copy OCs and whole proteins sequences, strain SM1903 fell within a sub-cluster encompassed by , and and formed a branch adjacent to . The major cellular fatty acids were summed feature 8 (C 7 and/or C 6), C and 11-methyl-C 7. The polar lipids mainly comprised phosphatidylglycerol, phosphatidylcholine, one unidentified lipid, one unidentified aminolipid, and one unidentified glycolipid. The solo respiratory quinone was ubiquinone-10. The genomic DNA G+C content of strain SM1903 was 66.0 mol%. Based on the results of phenotypic, chemotaxonomic, and phylogenetic characterization for strain SM1903, it is considered to represent a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is SM1903 (=MCCC 1K03608=KCTC 72046).
一株革兰氏阴性、需氧、卵圆形杆状细菌，被命名为 SM1903 菌株，从马里亚纳海沟的表层海水中分离得到。该菌株在 15-37°C（最适温度为 35°C）和 1-15%（最适盐度为 4%）的 NaCl 条件下生长。它能水解马栗树皮素，但不能将硝酸盐还原为亚硝酸盐，也不能水解吐温 80。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SM1903 形成了一个独立的谱系，与属内的种 （同源性最高为 95.7%）和 （同源性最高为 95.7%）的 16S rRNA 基因序列相似度最高。在基于单拷贝 OC 和全蛋白序列的系统发育树中，菌株 SM1903 位于 、 和 包含的亚群内，并与 形成了一个相邻的分支。主要的细胞脂肪酸为 sum feature 8 (C 7 和/或 C 6)、C 和 11-甲基-C 7。极性脂主要由磷脂酰甘油、磷脂酰胆碱、一种未鉴定的脂、一种未鉴定的胺脂和一种未鉴定的糖脂组成。唯一的呼吸醌为泛醌-10。菌株 SM1903 的基因组 DNA G+C 含量为 66.0 mol%。基于菌株 SM1903 的表型、化学分类和系统发育特征的研究结果，认为其代表了一个新属的新种，属于 科，建议命名为 属，新种，新种。模式菌株为 SM1903（=MCCC 1K03608=KCTC 72046）。
