Terada N, Takada T, Yamamoto R, Taniguchi H, Takatsuka D, Li W, Matsumoto K
Department of Experimental Pathology, Osaka University Medical School, Japan.
Exp Clin Endocrinol. 1989;94(3):227-32. doi: 10.1055/s-0029-1210903.
The production of oestrogen from androstenedione by ovaries of hamsters on day 14 of pregnancy and on days 0 and 3 after birth was investigated by culturing these ovaries for 48 h in serum-free medium containing insulin and [3H]androstenedione. Both oestrone and oestradiol-17 beta were produced in culture of ovaries on day 14 of pregnancy. The production of oestrogen increased about 2-fold in culture of ovaries on days 0 and 3. Dibutyryladenosine 3':5'-cyclic monophosphate stimulated the oestrogen production by ovaries on day 14 of pregnancy and on days 0 and 3. The present results indicate that the ovaries of fetal and neonatal hamsters have an aromatase activity and that these aromatase activities are responsive to adenosine 3':5'-cyclic monophosphate.
通过在含有胰岛素和[3H]雄烯二酮的无血清培养基中培养怀孕第14天以及出生后第0天和第3天的仓鼠卵巢48小时，研究了这些卵巢从雄烯二酮生成雌激素的情况。怀孕第14天的卵巢培养物中产生了雌酮和雌二醇-17β。在出生后第0天和第3天的卵巢培养物中，雌激素的生成增加了约2倍。二丁酰腺苷3':5'-环一磷酸刺激了怀孕第14天以及出生后第0天和第3天的卵巢生成雌激素。目前的结果表明，胎儿和新生仓鼠的卵巢具有芳香化酶活性，并且这些芳香化酶活性对腺苷3':5'-环一磷酸有反应。
